Analytical determination of the reducing and stabilization agents present in different Zostera noltii extracts used for the biosynthesis of gold nanoparticles
| Autor: | María J. Ortega, Ignacio Naranjo-Rodriguez, M.L.A. Gil, Juan José Delgado, Rafael Zarzuela, José María Palacios-Santander, Manuel Luna, Laura María Cubillana-Aguilera |
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| Rok vydání: | 2017 |
| Předmět: |
Magnetic Resonance Spectroscopy
Biophysics Molecular Conformation Nanoparticle Metal Nanoparticles 02 engineering and technology Mass spectrometry 01 natural sciences High-performance liquid chromatography Column chromatography Microscopy Electron Transmission Radiology Nuclear Medicine and imaging Particle Size Chromatography High Pressure Liquid Flavonoids Radiation Radiological and Ultrasound Technology 010405 organic chemistry Chemistry Plant Extracts Zosteraceae Spectrometry X-Ray Emission Green Chemistry Technology Nuclear magnetic resonance spectroscopy 021001 nanoscience & nanotechnology 0104 chemical sciences Electron diffraction Colloidal gold Transmission electron microscopy Gold 0210 nano-technology Nuclear chemistry |
| Zdroj: | Journal of photochemistry and photobiology. B, Biology. 179 |
| ISSN: | 1873-2682 |
| Popis: | The objective of this work was to ascertain the nature of the components responsible for the reducing and stabilizing properties of Zostera noltii extracts that lead to gold nanoparticle formation using chemical techniques of analysis. In order to achieve this aim, we try the synthesis of AuNPs with three different extracts from plants collected in the Bay of Cadiz (Spain). The n-butanol extract produced the best results. Taking this into account, four fractions were isolated by Sephadex LH-20 column chromatography from this extract and we studied their activity. The chemical study of these fractions led to the isolation of several flavone sulfates and these were identified as the species' responsible for the formation and stabilization of the AuNPs. Flavone sulfates were purified by high performance liquid chromatography and the structures were established by means of spectroscopic methods nuclear magnetic resonance and mass spectroscopy. AuNPs have an average lifetime of about 16weeks. Additionally, the morphology and crystalline phase of the gold nanoparticles were characterized by transmission electron microscopy. The composition of the nanoparticles was evaluated by electron diffraction and energy dispersive X-ray spectroscopy. An 88% of the gold nanoparticles has a diameter in the range 20-35nm, with an average size of 26±2nm. |
| Databáze: | OpenAIRE |
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