Involvement of TLR2 and TLR4 and Th1/Th2 shift in inflammatory responses induced by fine ambient particulate matter in mice

Autor:	Suxia Wang, Can Zhao, Tong-sheng Yang, Wei-li Chu, Ying-hong Tao, Ji-ping Liao, Guangfa Wang
Rok vydání:	2012
Předmět:	Male Pathology medicine.medical_specialty Health Toxicology and Mutagenesis Inflammation GATA3 Transcription Factor Toxicology Systemic inflammation Mice medicine Animals Particle Size Receptor Mice Inbred BALB C Lung medicine.diagnostic_test Chemistry Pneumonia Toll-Like Receptor 2 Toll-Like Receptor 4 TLR2 medicine.anatomical_structure Bronchoalveolar lavage TLR4 Cytokines Histopathology Environmental Pollutants Particulate Matter medicine.symptom T-Box Domain Proteins Bronchoalveolar Lavage Fluid
Zdroj:	Inhalation toxicology. 24(13)
ISSN:	1091-7691
Popis:	Epidemiologic studies have reported the association between fine particles (aerodynamic diameter ≤ 2.5 μm; PM2.5) and health effects, but the immunological mechanisms are not clear. To investigate the dose and time-dependent role of toll-like receptor (TLR) and Th1/Th2 shift in local and systemic inflammation induced by PM2.5, mice were subjected to intratracheal instillation of 2.5, 5, or 10 mg/kg PM2.5 in this study. After 24 h, 72 h, 7 days, and 14 days, mice were sacrificed to measure TLR2 and TLR4 expressions and Th1/Th2 related cytokines in bronchoalveolar lavage fluid (BALF) and peripheral blood. Histopathological changes in lung were also examined. Inflammatory infiltration and macrophages with engulfed particles were found by lung histopathology after PM2.5 exposure. TLR4 positive cells decreased in BALF but increased in blood at 24 h after the exposure. The low percentage of TLR4 positive cells continued to day 14 in BALF, but recovered at day 7 and decreased further to lower than the control value at day 14 in blood. TLR2 positive cell changed similar to TLR4 in BALF on the dose effects. In BALF at 24 h after the exposure, the Th2 related cytokines IL-5 and IL-10 increased dose-dependently; and in blood, the Th2 related cytokines IL-4, IL-5, and IL-10 also increased. These results suggest that acute exposure of PM2.5 leads to acute inflammatory responses locally and systemically in mice. TLR2 and TLR4 are involved in this process and PM2.5 can drive a Th2-biased immune response.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::037863346af3de27c075dfef23bd4c97 https://pubmed.ncbi.nlm.nih.gov/23121301 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
Nepřihlášeným uživatelům se plný text nezobrazuje	K zobrazení výsledku je třeba se přihlásit.