SFC-MS/MS as an orthogonal technique for improved screening of polar analytes in anti-doping control
Autor: | Maxi Wenzel, Mijo Stanic, Francesco Botrè, Maria Kristina Parr, Edgar Naegele, Jan Felix Joseph, Xavier de la Torre, Bernhard Wuest, Alexander H. Schmidt |
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Rok vydání: | 2016 |
Předmět: |
Resolution (mass spectrometry)
Dilute-and-inject Adrenergic beta-Antagonists Analytical chemistry 01 natural sciences Biochemistry High-performance liquid chromatography Analytical Chemistry Beta-blockers Limit of Detection Tandem Mass Spectrometry Humans Chromatography High Pressure Liquid Fenoterol Doping in Sports Chromatography 010405 organic chemistry Chemistry Elution Hydrophilic interaction chromatography 010401 analytical chemistry Doping control Polar stimulants Sulfoconjugates Sympathomimetic drugs Chromatography Supercritical Fluid Propranolol Supercritical fluid 0104 chemical sciences Bronchodilator Agents Solvent Substance Abuse Detection Pharmaceutical Preparations Supercritical fluid chromatography Polar |
Zdroj: | Analytical and bioanalytical chemistry. 408(24) |
ISSN: | 1618-2650 |
Popis: | HPLC is considered the method of choice for the separation of various classes of drugs. However, some analytes are still challenging as HPLC shows limited resolution capabilities for highly polar analytes as they interact insufficiently on conventional reversed-phase (RP) columns. Especially in combination with mass spectrometric detection, limitations apply for alterations of stationary phases. Some highly polar sympathomimetic drugs and their metabolites showed almost no retention on different RP columns. Their retention remains poor even on phenylhexyl phases that show different selectivity due to π-π interactions. Supercritical fluid chromatography (SFC) as an orthogonal separation technique to HPLC may help to overcome these issues. Selected polar drugs and metabolites were analyzed utilizing SFC separation. All compounds showed sharp peaks and good retention even for the very polar analytes, such as sulfoconjugates. Retention times and elution orders in SFC are different to both RP and HILIC separations as a result of the orthogonality. Short cycle times could be realized. As temperature and pressure strongly influence the polarity of supercritical fluids, precise regulation of temperature and backpressure is required for the stability of the retention times. As CO2 is the main constituent of the mobile phase in SFC, solvent consumption and solvent waste are considerably reduced. Graphical Abstract SFC-MS/MS vs. LC-MS/MS. |
Databáze: | OpenAIRE |
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