Stress enhances the sensitivity of Salmonella enterica serovar Typhimurium to bacteriocins
| Autor: | M.F. Galvão, Maria Cristina Dantas Vanetti, Cláudia Vieira Prudêncio |
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| Rok vydání: | 2015 |
| Předmět: |
Salmonella typhimurium
Salmonella Food industry Bovicin HC5 Hurdle technology Microbial Sensitivity Tests medicine.disease_cause Applied Microbiology and Biotechnology Microbiology chemistry.chemical_compound Trisodium phosphate Bacteriocin Stress Physiological medicine Food science Nisin biology Food preservation Biocontrol food and beverages General Medicine biochemical phenomena metabolism and nutrition biology.organism_classification Anti-Bacterial Agents Outer membrane chemistry Salmonella enterica bacteria Bacteria Biotechnology |
| Zdroj: | LOCUS Repositório Institucional da UFV Universidade Federal de Viçosa (UFV) instacron:UFV |
| ISSN: | 1364-5072 |
| DOI: | 10.1111/jam.12776 |
| Popis: | Aims The aim of this study was to evaluate the potential application of bacteriocins against Gram-negative bacteria when associated with others food preservation methods. Methods and Results Salmonella was subjected to heat, cold, acid and chemical (with ethylenediaminetetracetate and trisodium phosphate) stresses. Then, the cells were recovered and subjected to treatment with bacteriocins (500 AU ml−1) for 6 h. Heat and cold stress were those that promoted more sensitization to bactericidal activity of nisin. Under the same conditions, bovicin HC5 acted more rapidly than nisin reducing the number of viable cells to undetectable levels after 20 min of treatment. Similar results with use of nisin only were observed after 6 h of treatment. Conclusions Stress conditions used in food industry, such as temperature and pH, and use of chelating agents or membrane disruptors, sensitized Salmonella Typhimurium cells to bacteriocins produced by lactic acid bacteria, such as nisin and bovicin HC5. Significance and Impact of the Study Food preservation methods sensitized Gram-negative bacteria to bacteriocins activity, which demonstrate the potential of nisin and bovicin HC5 to inhibit the growth of Salmonella. |
| Databáze: | OpenAIRE |
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