Protein-based engineering of the initial acquired enamel pellicle in vivo: proteomic evaluation

Autor:	Tatiana Martini, Beatriz Martines de Souza, Talita Mendes Oliveira Ventura, Ana Luiza Bogaz Debortolli, Flávio Henrique-Silva, Priscila Yumi Tanaka Shibao, Caroline Fernanda da Silva Ribeiro, Aline Dionizio, Even Akemi Taira, Thamyris de Souza Carvalho, Reinaldo Marchetto, Marília Afonso Rabelo Buzalaf, Heloisa Aparecida Pereira Barbosa, Tamara Teodoro Araujo, Chelsea Maria Vilas Boas Feitosa
Přispěvatelé:	Universidade de São Paulo (USP), São Carlos Federal University, Universidade Estadual Paulista (UNESP)
Rok vydání:	2022
Předmět:	chemistry.chemical_classification Proteomics Proteins Water Peptide PROTEÍNAS CaneCPI-5 chemistry.chemical_compound chemistry Biochemistry In vivo Protein purification Keratin Humans Hemoglobin Cystatin Dental Pellicle Citric acid Peptides Acquired enamel pellicle General Dentistry Statherin
Zdroj:	Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP
Popis:	Made available in DSpace on 2022-04-29T08:36:23Z (GMT). No. of bitstreams: 0 Previous issue date: 2022-01-01 Objective: To study the proteomic alterations in the initial AEP after rinsing with CaneCPI-5, StN15 or Hb or their combination. Materials and methods: In five crossover phases, after prophylaxis, 10 volunteers in 5 consecutive days, rinsed (10 mL, 1 min) with the following solutions: deionized water (H2O- negative control- 1), 0.1 mg/mL CaneCPI-5 (2), 1.88×10−5 M StN15 (3), 1.0 mg/mL Hb (4) or their combination (5). The AEP formed after 3 min was collected with electrode filter papers soaked in 3% citric acid. After protein extraction, samples were analyzed by quantitative shotgun label-free proteomics. Results: Rinsing with the proteins/peptide increased the amounts of proteins in the AEP. The total numbers of proteins identified after rinsing with CaneCPI-5, StN15, Hb or their combination versus water, were 131, 167, 148 and 142, respectively. The treatment with the proteins/peptide or their combination increased proteins that bind calcium, phosphate and interact with distinct proteins, as well as proteins with antimicrobial and acid-resistant properties, such as, Cornifin-B (7.7, 12.6, and 4.3-fold for CaneCPI-5, StN15 and Hb, respectively), isoforms of Cystatin (2.2–2.4-fold for CaneCPI-5 and StN15), Proline-rich-protein 4 (4.3-fold; StN15), Histatin-1 (2.8-fold; StN15) and Hemoglobin (7.7–25-fold for Hb and Combination). Immunoglobulin, Keratin and Histone were exclusively identified upon treatment with the proteins/peptide, alone or combined. Conclusion: Rinsing with proteins/peptide, alone or combined, increased protective proteins in the initial AEP. Clinical Relevance: Our results suggest that rinsing with the proteins/peptide or their combination increases the proteins capable of enhancing the protective function of the basal layer of AEP. Department of Biological Sciences Bauru School of Dentistry University of São Paulo Department of Genetics and Evolution São Carlos Federal University Department of Biochemistry and Technology Institute of Chemistry São Paulo State University (UNESP) Department of Biochemistry and Technology Institute of Chemistry São Paulo State University (UNESP)
Databáze:	OpenAIRE
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