| Autor: |
Deniz Özen, A.G. Akkan, Yasemin Gizem Ozer, Bülent Demir, Cemre Kandaz, Burak Önal |
| Přispěvatelé: |
İstinye Üniversitesi, Mühendislik ve Doğa Bilimleri Fakültesi, Moleküler Biyoloji ve Genetik Bölümü, Ozen, Deniz, İÜC, Cerrahpaşa Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Tıp Fakültesi |
| Jazyk: |
angličtina |
| Rok vydání: |
2019 |
| Předmět: |
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| Popis: |
Ozen, Deniz/0000-0002-3095-1208 WOS:000516543400006 Objective: Cardiac syndrome X (CSX) can be defined as experiencing chest pain with angina by patients with normal coronary arteries. Endothelial and microvascular dysfunction is responsible for the pathophysiology of the inflammatory CSX disease. Inflammation is especially an important factor in the progression of ischemic heart diseases. It is accepted that both the number of B and T lymphocytes and expression of various proinflammatory cytokines released from those cells increases during inflammatory response. Different proinflammatory cytokines are produced by activated macrophages and T lymphocytes. Since interleukin-17 (IL-17) induces many signaling molecules, which promote immune response and play a role in inflammation, it is assumed that IL-17 can play a role in the CSX pathogenesis. In our study, the relationship between CSX and IL-17 serum levels and the -152G/A polymorphism on IL-17 gene was investigated. Methods: Serum IL-17 levels of blood samples were analyzed from 100 patients with CSX and 101 healthy control individuals using the Enzyme-Linked ImmunoSorbent Assay (ELISA) method. After the DNA isolation was performed from blood samples, the IL-17 gene-152G/A polymorphisms were detected based on the PCR-RFLP method for both patients and healthy individuals. The pomotor region of the IL-17 gene was amplified by the PCR method, and then, genotyping was performed using PCR products for the RFLP step. After that, obtained data for CSX patients and the healthy control group were compared using the statistical analysis. Results: When the IL-17 gene-152G/A polymorphism genotyping results of patients with CSX and healthy control individuals were compared, no statistically significant difference was observed in both genotypic and allelic distributions (p=0.218). The IL-17 serum levels were found to be significantly higher in patients with CSX than in healthy controls (p |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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