| Autor: |
Anthony J. Chalmers, Sidhartha A. Dongre, Katrina H. Stevenson, Mark R. Jackson, Seamus P. Caragher, Sandeep K. Chahal, Ming Y Chong, Natividad Gomez-Roman |
| Rok vydání: |
2023 |
| DOI: |
10.1158/1535-7163.22510557 |
| Popis: |
Erlotinib radiosensitisation of 2D GSC is VEGF-independent. A-D, Clonogenic survival of R10 GSC grown in 3D (A) and 2D (B-D) conditions and irradiated with single doses of X-rays (0-6 Gy; n=3) 2 hours after treatment of erlotinib (1microM) or vehicle (DMSO) in the presence (+VEGF) or absence (-VEGF) of VEGF (3 ng/ml). Mean {plus minus} SD of 3 independent experiments is shown; curves are fitted to a linear quadratic model. Erlotinib significantly radioprotected 3D VEGF-deprived cells (two-way ANOVA; R10 3D (-) VEGF vehicle vs 3D (-) VEGF plus erlotinib p < 0.05). No significant effect of erlotinib was observed in the presence of VEGF in 3D cells. Erlotinib radiosensitised 2D G7, E2 and R10 cells irrespective of VEGF presence (B-D). E, Quantification of gH2AX foci per nucleus in G7 3D cells treated with erlotinib in the absence of VEGF, or with VEGF after radiation treatment (24 h). Graph represents mean of medians from 3 independent experiments. p values calculated by t test (* p |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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