Volatile substances in blood serum: profile analysis and quantitative determination
| Autor: | H.M. Liebich, J. Wöll |
|---|---|
| Rok vydání: | 1977 |
| Předmět: |
Aldehydes
Ethanol Volatilisation Chromatography Computers Organic Chemistry Extraction (chemistry) General Medicine Ketones Biochemistry Quantitative determination Gas Chromatography-Mass Spectrometry Analytical Chemistry chemistry.chemical_compound Blood serum chemistry Acetone Humans Profile analysis Gas chromatography–mass spectrometry Volatilization |
| Zdroj: | Journal of chromatography. 142 |
| Popis: | Alcohols, ketones and aldehydes have been identified in the profiles of volatile substances in blood serum. A 5-ml sample is required in order to obtain a complete gas chromatographic profile, selective profiles of alcohols or individual ketones by computer mass fragmentography, and to permit mass spectrometric identification of the compounds. The quantitative determination of total 4-heptanone using an extraction and gas chromatographic-mass fragmentographic procedure demonstrated concentrations of 10-50 nmole/l in normal serum, whereas in the serum of patients with chronic renal insufficiency the concentrations were 10- to 40-fold higher. Ethanol and total acetone were quantitatively measured by direct injection of serum. For 10 patients, concentrations between 25 and 85 mumole/l of acetone and 10-170 mumole/l of ethanol were determined. |
| Databáze: | OpenAIRE |
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