Gene expression of Na+/Ca2+ exchanger during development in human heart
| Autor: | Mohamed Boutjdir, Nabil El-Sherif, Yongxia Qu, Ashwini Ghatpande |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
medicine.medical_specialty
Physiology Blotting Western Biology Sodium-Calcium Exchanger Embryonic and Fetal Development Fetal Heart Sarcolemma Western blot Pregnancy Physiology (medical) Internal medicine Gene expression medicine Myocyte Humans RNA Messenger Fluorescent Antibody Technique Indirect Fetus Messenger RNA Immature animal Microscopy Confocal medicine.diagnostic_test Gene Expression Regulation Developmental Nuclease protection assay Pregnancy Trimester First Endocrinology Pregnancy Trimester Second Gestation Female Cardiology and Cardiovascular Medicine Densitometry |
| Zdroj: | Cardiovascular research. 45(4) |
| ISSN: | 0008-6363 |
| Popis: | Objective: In immature animal hearts, lower activity of sarcoplasmic reticulum and lower densities of Ca2+ channels highlight the potentially vital role of the Na+/Ca2+ exchanger (NCX) to excitation–contraction coupling. To date, studies on NCX expression have been restricted to late developmental stages. The distribution and gene expression of NCX during early ontogeny is not known, especially in humans. In the present report, we systematically characterized changes in NCX gene expression in human heart during development, with particular emphasis in early ontogeny. Methods: Human hearts during early gestation (9- to 20-week gestation), neonatal (1 to 2 days after birth) and adulthood (18–40 years old) were used. NCX mRNA levels were studied using RNase Protection Assay (RPA) and NCX protein levels were assessed by Western blot. Wet weight was also used as the tissue base. Immunolocalization studies using confocal microscopy were performed in isolated fetal cardiac myocytes. Results: Normalization of NCX mRNA derived from ventricles against an early gestational age (10-week gestation) shows that NCX mRNA levels nominally increased from 1 to 1.13 at 19-week gestation then decreased to 0.74 ( P |
| Databáze: | OpenAIRE |
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