Quantitative evaluation of bone resorption activity of osteoclast-like cells by measuring calcium phosphate resorbing area using incubator-facilitated and video-enhanced microscopy
| Autor: | Akira Nagano, Susumu Terakawa, Hironobu Hoshino, Takashi Sakurai, Yoshitaka Morimoto |
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| Rok vydání: | 2008 |
| Předmět: |
Calcitonin
Calcium Phosphates Male Histology chemistry.chemical_element Osteoclasts Bone Marrow Cells Calcium Bone resorption Mice Osteoclast Elcatonin medicine Animals Microscopy Phase-Contrast Bone Resorption Instrumentation Microscopy Video Chemistry Anatomy In vitro Coculture Techniques Cell biology Medical Laboratory Technology medicine.anatomical_structure Video enhanced microscopy Bone marrow medicine.drug |
| Zdroj: | Microscopy research and technique. 72(4) |
| ISSN: | 1097-0029 |
| Popis: | Quantitative evaluation of the ability of bone resorption activity in live osteoclast-like cells (OCLs) has not yet been reported on. In this study, we observed the sequential morphological change of OCLs and measured the resorbing calcium phosphate (CP) area made by OCLs alone and with the addition of elcatonin utilizing incubator facilitated video-enhanced microscopy. OCLs, which were obtained from a coculture of ddy-mouse osteoblastic cells and bone marrow cells, were cultured on CP-coated quartz cover slips. The CP-free area increased constantly in the OCLs alone, whereas it did not increase after the addition of elcatonin. This study showed that analysis of the resorbed areas under the OCL body using this method enables the sequential quantitative evaluation of the bone resorption activity and the effect of several therapeutic agents on bone resorption in vitro. Microsc. Res. Tech, 2009. © 2008 Wiley-Liss, Inc. |
| Databáze: | OpenAIRE |
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