Hyperthyroidism in the developing rat testis is associated with oxidative stress and hyperphosphorylated vimentin accumulation
| Autor: | Fátima Theresinha Costa Rodrigues Guma, Viviane Mara Woehl, Fabíola Sell, Kátia Padilha Barreto, Fátima Regina Mena Barreto Silva, Regina Pessoa-Pureur, Ariane Zamoner, Danilo Wilhelm Filho |
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| Rok vydání: | 2006 |
| Předmět: |
Mitochondrial ROS
Male medicine.medical_specialty Thyroid Hormones Antioxidant medicine.medical_treatment Vimentin Biology medicine.disease_cause Biochemistry Hyperthyroidism Lipid peroxidation chemistry.chemical_compound Endocrinology Oxygen Consumption Internal medicine Testis medicine TBARS Animals RNA Messenger Phosphorylation Rats Wistar Extracellular Signal-Regulated MAP Kinases Molecular Biology Kinase Body Weight Glutathione Organ Size Enzymes Rats Oxidative Stress chemistry Gene Expression Regulation Glycoprotein Hormones alpha Subunit biology.protein Lipid Peroxidation Oxidative stress |
| Zdroj: | Molecular and cellular endocrinology. 267(1-2) |
| ISSN: | 0303-7207 |
| Popis: | Hyperthyroidism was induced in rats and somatic indices and metabolic parameters were analyzed in testis. In addition, the morphological analysis evidenced testes maturation and intense protein synthesis and processing, supporting the enhancement in vimentin synthesis in hyperthyroid testis. Furthermore, vimentin phosphorylation was increased, indicating an accumulation of phosphorylated vimentin associated to the cytoskeleton, which could be a consequence of the extracellular-regulated kinase (ERK) activation regulating the cytoskeleton. Biomarkers of oxidative stress demonstrated an increased basal metabolic rate measured by tissue oxygen consumption, as well as, increased TBARS levels. In addition, the enzymatic and non-enzymatic antioxidant defences appeared to respond according to the augmented oxygen consumption. We observed decreased total glutathione levels, with enhancement of reduced glutathione, whereas most of the antioxidant enzyme activities were induced. Otherwise, superoxide dismutase activity was inhibited. These results support the idea that an increase in mitochondrial ROS generation, underlying cellular oxidative damage, is a side effect of hyperthyroid-induced biochemical changes by which rat testis increase their metabolic capacity. |
| Databáze: | OpenAIRE |
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