Stem Cell Therapy Improves Myocardial Perfusion and Cardiac Synchronicity: New Application for Echocardiography
| Autor: | Ulf Krause, David Wolf, Alexander Hansen, Adrian Reinhard, Hugo A. Katus, Helmut F. Kuecherer, Anja Seckinger |
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| Rok vydání: | 2007 |
| Předmět: |
medicine.medical_specialty
Swine Heart Ventricles medicine.medical_treatment Myocardial Infarction Contrast Media Anterior Descending Coronary Artery Mesenchymal Stem Cell Transplantation Coronary Circulation Internal medicine Occlusion medicine Animals Radiology Nuclear Medicine and imaging Myocardial infarction Allogeneic mscs Myocardial tissue business.industry Mesenchymal stem cell Stem-cell therapy medicine.disease Myocardial Contraction Disease Models Animal Treatment Outcome Echocardiography Injections Intravenous Cardiology Cardiology and Cardiovascular Medicine business Perfusion Follow-Up Studies |
| Zdroj: | Journal of the American Society of Echocardiography. 20:512-520 |
| ISSN: | 0894-7317 |
| Popis: | Intravenous delivery of mesenchymal stem cell (MSC) is a noninvasive approach for myocardial tissue repair. We aimed to test this strategy in a pig model of myocardial infarction and to examine the usefulness of new echocardiographic applications to monitor cardioprotective effects of stem cell therapy.Pigs (n = 8) received autologous or allogeneic MSCs (1 x 10(6)/kg body weight) labeled with fluorescent dye 48 hours after proximal left anterior descending coronary artery occlusion. Infarct size, myocardial function, and perfusion (A x beta) were assessed by myocardial contrast echocardiography and standard histologic methods after 1 month.Morphologic analysis revealed that labeled MSCs migrated in the peri-infarct region resulting in smaller infarct size by myocardial contrast echocardiography (control vs autologous and allogeneic MSC: 38 +/- 10% vs 25 +/- 5% and 28 +/- 6%, P.01), higher fractional area shortening (23 +/- 3% vs 34.0 +/- 7% and 28 +/- 2%, P.01), higher cardiac synchrony (167 +/- 36 vs 68 +/- 17 and 85 +/- 26 milliseconds, P.003), and improved microvascular flow A x beta in the ischemic border zone (0.18 +/- 0.2 vs 0.56 +/- 0.3 and 0.49 +/- 0.2, P.03).Systemic delivery of autologous and allogeneic MSCs preserves myocardial viability even in large animals and is, therefore, an attractive approach for tissue repair. Myocardial contrast echocardiography is useful to evaluate microvascular perfusion, which was enhanced by MSCs. |
| Databáze: | OpenAIRE |
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