Beta-nerve growth factor stimulates spontaneous electrical activity of in vitro embryonic mouse GnRH neurons through a P75 mediated-mechanism
| Autor: | Catherine Taragnat, Caroline Pinet-Charvet, Renaud Fleurot, Thierry Moreau, Guillaume Tsikis, Xavier Druart, Valérie Labas, Ana-Paula Teixeira-Gomes, Anne Duittoz, Flavie Derouin-Tochon, Xavier Cayla, Simon P. de Graaf |
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| Přispěvatelé: | Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), School of Life and Environmental Sciences A08 [Australia) (University of Sydney), The University of Sydney, Infectiologie et Santé Publique (UMR ISP), Université de Tours (UT)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Biologie des Oiseaux et Aviculture (BOA), INRA PHASE department (IMPAIC project), IFCE (NOVO project), Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Université de Tours-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) |
| Rok vydání: | 2020 |
| Předmět: |
Male
INVOLVEMENT 0301 basic medicine Physiology [SDV]Life Sciences [q-bio] Beta-Nerve Growth Factor lcsh:Medicine Gonadotropin-Releasing Hormone Mice GABA Endocrinology 0302 clinical medicine Induced ovulation Nerve Growth Factor OVULATION-INDUCING FACTOR lcsh:Science Receptor media_common Neurons GONADOTROPIN-RELEASING-HORMONE Multidisciplinary Reproduction Neurotrophic factors ENSHEATHING CELLS Recombinant Proteins Cell biology SECRETION Female [SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC] Ovulation media_common.quotation_subject Reproductive biology STATISTICAL-MODEL Biology Article 03 medical and health sciences Ovulation Induction Corpus Luteum Semen In vivo Animals Humans [SDV.BA.MVSA]Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health SEMINAL PLASMA lcsh:R [SDV.BDLR]Life Sciences [q-bio]/Reproductive Biology Embryonic stem cell In vitro 030104 developmental biology nervous system Central Nervous System Stimulants lcsh:Q HYPOTHALAMUS Olfactory ensheathing glia BIOLOGICAL-ACTIVITY 030217 neurology & neurosurgery |
| Zdroj: | Scientific Reports, Vol 10, Iss 1, Pp 1-14 (2020) Scientific Reports Scientific Reports, Nature Publishing Group, 2020, 10 (1), 14 p. ⟨10.1038/s41598-020-67665-4⟩ |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/s41598-020-67665-4 |
| Popis: | The control of ovulation helps guarantee the success of reproduction and as such, contributes to the fitness of a species. In mammals, two types of ovulation are observed: induced and spontaneous ovulation. Recent work on camelids, that are induced ovulators, highlighted the role of a factor present in seminal plasma, beta Nerve Growth Factor (β-NGF), as the factor that triggers ovulation in a GnRH dependent manner. In the present work, we characterized alpaca β-NGF (aβ-NGF) and its 3D structure and compared it with human recombinant β-NGF (hβ-NGF). We showed that the β-NGF enriched fraction of alpaca semen and the human recombinant protein, both stimulated spontaneous electrical activity of primary GnRH neurons derived from mouse embryonic olfactory placodes. This effect was dose-dependent and mediated by p75 receptor signaling. P75 receptors were found expressed in vitro by olfactory ensheathing cells (OEC) in close association with GnRH neurons and in vivo by tanycytes in close vicinity to GnRH fibers in adult mouse. Altogether, these results suggested that β-NGF induced ovulation through an increase in GnRH secretion provoked by a glial dependent P75 mediated mechanism. |
| Databáze: | OpenAIRE |
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