| Popis: |
PDF file - 701KB, Supplementary Figure S6. (A) 1) Expression of the depleted genes, RASAL2 and NENF, quantified by QPCR after first (I) and second (II) transfection of Hep3B HCC cell line with scrambled siRNA (siCtrl) and siRNA directed to RASAL2 (siRASAL2) or NENF (siNENF). 2) Effect on cell growth after first (day 3), second (day 6) and third (day 9) transfection with siRASAL2 or siNENF. 3) Effect on cell invasion as measured by Boyden chamber invasion assay after second transfection with siRASAL2 or siNENF. For Hep3B cells transfected with siRASAL2 or siNENF, evaluation of expression after third transfection was not possible due to the reduced cell number after three transfections. Therefore, invasive assay measurements were performed after second transfection. All results represent mean � S.D. of two or three determinations in either two or three independent experiments - ***P < 0.001, **P < 0.01, *P < 0.05. (B) HepG2 and SkHep1 cells were transfected twice with siCtrl, siRASAL2 or siNENF followed by subcutaneous injection of 2x106 HepG2 and 1x106 SkHep1 cells into the NOD/SCID mice as described in the Materials and Methods. Each group was composed of six male mice 4-6 weeks old. Differences in tumor volume were monitored starting from post-injection week 2 until the mice were sacrificed and are demonstrated in charts. Results represent the mean � SEM for each group of animals - ***P < 0.001, **P < 0.01, *P < 0.05. |
