A flow-based enzyme-free biosensor fabricated using track-etched membrane electrodes : Selective and sensitive detection of uric acid
| Autor: | Hitoshi Mizuguchi, Soma Fujiki, Takaki Shibata, Masatsugu Oishi, Masamitsu Iiyama, Toshio Takayanagi, Yu-Chi Lin, Min-Hsin Yeh |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2023 |
| Předmět: |
Enzyme-free electrochemical biosensor
2-aminoethanethiol Acetylene black Materials Chemistry Metals and Alloys Electrical and Electronic Engineering Track-etched membrane electrode Condensed Matter Physics Instrumentation Uric acid Surfaces Coatings and Films Electronic Optical and Magnetic Materials |
| Zdroj: | Sensors and Actuators B : Chemical. 383:133588 |
| ISSN: | 0925-4005 |
| Popis: | A flow-based, enzyme-free biosensor for the selective and sensitive determination of uric acid was developed using track-etched membrane electrodes (TEMEs). The proposed sensor system comprises a pre-reactor electrode to decompose interfering species, such as L-ascorbic acid, and a detector electrode to detect uric acid. TEMEs were prepared by sputter deposition of gold on a track-etched membrane filter. Acetylene black (AB) was introduced as an electrode catalyst by simple filtration with the TEME, and the sensitivity for uric acid using the AB-decorated Au-TEME was much greater than that with bare Au-TEME. The insertion of the 2-aminoethanethiol-modified Au-TEMEs as pre-reactor electrodes upstream of the AB-decorated detector electrode eliminated the interference caused by the co-existence of L-ascorbic acid because the interfering species were electrolyzed at the pre-reactor electrode prior to detection of uric acid. The proposed sensor system was incorporated into a flow-injection analysis system to evaluate the sensor performance. The detection limit of uric acid was 0.6 μM. A recovery test by standard addition to human urine samples also showed satisfactory results. |
| Databáze: | OpenAIRE |
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