Rational Design of Hypoallergenic Vaccines: Blocking IgE-binding to Polcalcin Using Allergen-specific IgG Antibodies
| Autor: | Faezeh Soltanpour, Gholamreza Khamisipour, Mohsen Mohammadi, Niloofar Momenzadeh, Fatemeh Omrani, Behrooz Taheri, Moradali Fouladvand |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Adult
Male lcsh:Medicine Peripheral blood mononuclear cell Epitope Chenopodium album law.invention Immune system Antigen law Animals Humans Immunology and Allergy Mice Inbred BALB C biology Chemistry lcsh:R Calcium-Binding Proteins Rhinitis Allergic Seasonal Hypoallergenic Allergens Antigens Plant Immunoglobulin E Middle Aged Polcalcin Molecular biology Immunoglobulin G Recombinant fusion proteins Vaccines Subunit biology.protein Recombinant DNA Female Immunotherapy Antibody Peptides Vaccine Keyhole limpet hemocyanin |
| Zdroj: | Iranian Journal of Allergy, Asthma and Immunology, Vol 19, Iss 3 (2020) |
| ISSN: | 1735-5249 1735-1502 |
| DOI: | 10.18502/ijaai.v19i3.3455 |
| Popis: | Chenopodium album polcalcin (Che a 3) is characterized as a major cause of cross-reactivity inallergic patients to the Chenopodiaceae family. Therefore, the present study was conducted to develop a hypoallergenic Che a 3 derivatives as the candidate vaccine for type 1 allergy. Four derivatives were generated from Che a 3. The first was a mosaic peptide derivative computationally identified in Che a 3 which was coupled to keyhole limpet hemocyanin (KLH). The second one was a mutant Che a 3, and the other two derivatives included N- and C-terminal halves of Che a 3 that both coupled to KLH. The IgE-binding capacity of Che a 3 and its derivatives and also their ability to induce there combinant Che a 3 (rChe a 3)-specific IgG antibody, were determined using the enzyme-linked immune sorbent assay (ELISA). Moreover, the lymphopro liferative capacity of rChe a 3 or its derivatives and their pro-inflammatory cytokine response interleukin (IL)-5 and IL-13 were measured in the human peripheral blood mononuclear cells (PBMCs). Among all derivatives, the N-terminal half peptide and mosaic peptide exhibited the lowest IgE-binding capacity. In addition, in comparison to other antigens, KLH-coupled mosaic peptide induced the highest level of the recombinant Che a 3 (rChe a 3)-specific IgG antibody and ther Che a 3 specific-blocking IgG antibody in mice. Moreover, the mosaic peptide lacked lymphopro liferative capacity and down-regulated expression of pro-allergic IL-5 and IL-13 cytokines. Therefore, a peptide-carrier fusion vaccine, composed of the B-cell epitope coupled to the carrier, could be considered as one of the promising hypoallergenic vaccines to treat patients with allergy to low molecular weight allergens such as Che a 3. |
| Databáze: | OpenAIRE |
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