Performance of an Immunochromatographic Test (ICT) in Comparison to Some Commonly Used Serological Tests for the Diagnosis of Brucellosis in Dromedary Camels (Camelus dromedarius)
| Autor: | Fabrizio De Massis, Asma M Al Mansoori, Abdelmalik I. Khalafalla, Salama S Almuhairi, Mariam S Alketbi, Armando Giovannini, Saleha A Al Jaberi, Wissam S Serhan, Mohamed A Al Hosani, Paolo Calistri, Asma S Al Ketbi, Esmat F Gasim, Rashid Khan |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Microbiology (medical)
serological tests Veterinary medicine endocrine system 040301 veterinary sciences Immunochromatographic test Microbiology Article Serology 0403 veterinary science 03 medical and health sciences Virology medicine lcsh:QH301-705.5 030304 developmental biology 0303 health sciences business.industry Significant difference Brucellosis 04 agricultural and veterinary sciences dromedary camels medicine.disease brucellosis diagnosis lcsh:Biology (General) business performance |
| Zdroj: | Microorganisms Volume 7 Issue 12 Microorganisms, Vol 7, Iss 12, p 591 (2019) |
| ISSN: | 2076-2607 |
| DOI: | 10.3390/microorganisms7120591 |
| Popis: | Serological tests may represent an essential tool for the diagnosis of camel brucellosis however, concerns arise in the scientific community regarding the direct transposition from cattle and small ruminants without adequate validation. The present study was made to compare four serological tests for the diagnosis of brucellosis in dromedary camels (Camelus dromedarius). In terms of sensitivity, our results show that the Immunochromatographic Test (ICT) shows the higher value of sensitivity, 98.67% (95% Confidence Level (C.L): 94.36%&ndash 99.99%), followed by the Fluorescence Polarization Assay (FPA) with 95.05% (95% C.L: 88.23%&ndash 99.51%), then the Competitive Enzyme-Linked Immunosorbent Assay (c-ELISA) with 94.94% (95% C.L: 88.25%&ndash 99.45%) and, finally, the Rose Bengal Test (RBT) with 68.95% (95% C.L: 56.55%&ndash 80.69%), which is the only test showing a significantly lower sensitivity compared to the others. On the other hand, our study revealed no significant difference in terms of specificity between all the tests under study, with a range from 99.06% (95% C.L: 98.34%&ndash 99.64%) for the ICT to 99.92% (95% C.L: 99.64%&ndash 100%) for the RBT. The ICT was found to be comparable in terms of sensitivity and specificity with the most commonly used tests for camel brucellosis. The results of the present study are of paramount importance for designing surveillance and control measures for brucellosis in camel populations. |
| Databáze: | OpenAIRE |
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