Characterisation and Gene Expression Profiling of a Stable Cell Line Expressing a Cell Cycle GFP Sensor
| Autor: | Gretchen L. Kiser, Mike Kenrick, Simon Laurence John Stubbs, Hayley Tinkler, Nicholas Thomas, Theresa L. Giesler |
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| Rok vydání: | 2004 |
| Předmět: |
G2 Phase
Cell division Recombinant Fusion Proteins Green Fluorescent Proteins Cell Cell Cycle Proteins Biology Cell cycle phase Green fluorescent protein Cell Line Tumor Cyclins medicine Humans RNA Messenger Cell Cycle Protein Molecular Biology Reverse Transcriptase Polymerase Chain Reaction Cell growth Gene Expression Profiling Cell Cycle DNA Cell Biology Cell cycle Flow Cytometry Microarray Analysis Molecular biology Cell biology medicine.anatomical_structure Gene Expression Regulation Cell culture Cell Division Developmental Biology |
| Zdroj: | Cell Cycle. 4:191-195 |
| ISSN: | 1551-4005 1538-4101 |
| DOI: | 10.4161/cc.4.1.1405 |
| Popis: | The use of stable cell lines expressing fusions with green fluorescent protein (GFP) has increased significantly in recent years. In this study we have used a range of complimentary analytical techniques to examine the characteristics of a cell line stably expressing a EGFP cell cycle sensor relative to parental U2OS cells. Analysis of cell cycle duration and cell cycle phase distribution by cell growth assays and flow cytometry revealed that the two cell lines had identical doubling times and cell cycle distributions. Measurement of EGFP fusion protein mRNA by quantitative RT-PCR indicated a EGFP sensor expression level equivalent to endogenous Cyclin B1 (7000 copies/cell in G2). Microarray analysis showed a 0.9% (>2 fold at p |
| Databáze: | OpenAIRE |
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