MicroRNA-886-3P functions as a tumor suppressor in small cell lung cancer
| Autor: | Jie Shen, Wei Zhou, Qi-Min Zhan, Lv-Hua Wang, Nan Bi, Yong-Mei Song, Fu-Quan Zhang |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Cancer Research Epithelial-Mesenchymal Transition Lung Neoplasms Cell Gene Expression Treatment of lung cancer law.invention Transforming Growth Factor beta1 Mice 03 medical and health sciences 0302 clinical medicine Cell Movement Genes Reporter In vivo law Cell Line Tumor microRNA medicine Animals Humans Genes Tumor Suppressor Lung cancer 3' Untranslated Regions Cell Proliferation Pharmacology Chemistry Cell growth Transfection medicine.disease Immunohistochemistry Small Cell Lung Carcinoma Xenograft Model Antitumor Assays humanities Tumor Burden respiratory tract diseases Gene Expression Regulation Neoplastic Disease Models Animal MicroRNAs Phenotype 030104 developmental biology medicine.anatomical_structure Oncology 030220 oncology & carcinogenesis Cancer research Molecular Medicine Suppressor RNA Interference Research Paper |
| Zdroj: | Cancer Biology & Therapy. 19:1185-1192 |
| ISSN: | 1555-8576 1538-4047 |
| Popis: | Small cell lung cancer (SCLC) is a highly aggressive disease and miRNAs may play an important role in modulating SCLC progression. We have previously screened 924 miRNAs and found that miR-886-3P was negatively associated with SCLC survival. In the current study, we further investigated the role of miR-886-3P mimic in regulating SCLC cell phenotypic alteration in vitro and xenograft tumor formation in vivo. We found that transfection of miR-886-3P mimic significantly inhibited SCLC cell proliferation, migration, and colony formation, and induced mesenchymal-epithelial transition (MET) by suppressing TGF-ß1 synthesis in vitro. Furthermore, intra-tumor injection of miR-886-3P mimic lead to necrosis and suppression of tumor invasion to the surrounding tissue in the subcutaneous xenograft tumor, and intra-vein injection of miR-886-3P mimic suppressed xenograft lung cancer growth in vivo. These findings suggested that miR-886-3P functions as a tumor suppressor in SCLC and thus, it might be a potential therapeutic molecule in the treatment of lung cancer. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|