Fast label-free detection of C-reactive protein using broad-band Mach-Zehnder interferometers integrated on silicon chips
Autor: | Dimitrios Goustouridis, Sotirios E. Kakabakos, Gerhard Jobst, Eleni Makarona, Nikolaos Chaniotakis, Gerasimos Stefanitsis, Alexandros Salapatas, A. Psarouli, Konstantinos Misiakos, Ioannis Raptis, A. Botsialas, Dimitra Nikita, Panagiota S. Petrou |
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Rok vydání: | 2017 |
Předmět: |
Detection limit
Silicon Analyte Dynamic range Chemistry 010401 analytical chemistry Analytical chemistry Linearity Biosensing Techniques Equipment Design 02 engineering and technology Repeatability 021001 nanoscience & nanotechnology Mach–Zehnder interferometer 01 natural sciences 0104 chemical sciences Analytical Chemistry Dilution C-Reactive Protein Interferometry Limit of Detection Astronomical interferometer Humans 0210 nano-technology |
Zdroj: | Talanta. 165:458-465 |
ISSN: | 0039-9140 |
Popis: | An immunosensor for fast and accurate determination of C-reactive protein (CRP) in human serum samples based on an array of all-silicon broad-band Mach-Zehnder interferometers (BB-MZIs) is demonstrated. The detection was based on monitoring the spectral shifts during the binding of CRP on the antibody molecules that have been immobilized on the sensing arms of the BB-MZIs. By employing the reaction rate as the analytical signal the assay time was compressed to few minutes. The detection limit was 2.1ng/mL, the quantification limit was 4.2ng/mL and the linear dynamic range extended up to 100ng/mL. The measurements performed in human serum samples with the developed immunosensor were characterized by high repeatability and accuracy as it was demonstrated by dilution linearity and recovery experiments. In addition, the concentration values determined were in excellent agreement with those determined for the same samples by a standard clinical laboratory method. The compact size of the chip makes the proposed immunosensor attractive for incorporation into miniaturized devices for the determination of clinical analytes at the point-of-need. |
Databáze: | OpenAIRE |
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