Detailed Characterization of Human Induced Pluripotent Stem Cells Manufactured for Therapeutic Applications
Autor: | Mahendra S. Rao, Thomas Fellner, Ying Pei, Xianmin Zeng, Adhikarla Syama, Renuka Sivapatham, Odity Mukherjee, Behnam Ahmadian Baghbaderani |
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Jazyk: | angličtina |
Předmět: |
0301 basic medicine
Pluripotent Stem Cells Embryonic stem cells Cancer Research Cellular differentiation Induced Pluripotent Stem Cells Karyotype Gene Expression Antigens CD34 Computational biology Embryoid body Tissue Banks Biology Stem cell marker Polymorphism Single Nucleotide Article Cell Line Consent 03 medical and health sciences Humans Induced pluripotent stem cell Cells Cultured Embryoid Bodies Cell Proliferation Genetics Markers Comparative Genomic Hybridization Genome Human Cell Differentiation Sequence Analysis DNA Cell Biology Fetal Blood Flow Cytometry Embryonic stem cell Immunohistochemistry 3. Good health cGMP Manufacturing 030104 developmental biology Human genome Stem cell Comparative genomic hybridization Stem Cell Transplantation Developmental Biology |
Zdroj: | Stem Cell Reviews |
ISSN: | 1550-8943 |
DOI: | 10.1007/s12015-016-9662-8 |
Popis: | We have recently described manufacturing of human induced pluripotent stem cells (iPSC) master cell banks (MCB) generated by a clinically compliant process using cord blood as a starting material (Baghbaderani et al. in Stem Cell Reports, 5(4), 647–659, 2015). In this manuscript, we describe the detailed characterization of the two iPSC clones generated using this process, including whole genome sequencing (WGS), microarray, and comparative genomic hybridization (aCGH) single nucleotide polymorphism (SNP) analysis. We compare their profiles with a proposed calibration material and with a reporter subclone and lines made by a similar process from different donors. We believe that iPSCs are likely to be used to make multiple clinical products. We further believe that the lines used as input material will be used at different sites and, given their immortal status, will be used for many years or even decades. Therefore, it will be important to develop assays to monitor the state of the cells and their drift in culture. We suggest that a detailed characterization of the initial status of the cells, a comparison with some calibration material and the development of reporter sublcones will help determine which set of tests will be most useful in monitoring the cells and establishing criteria for discarding a line. Electronic supplementary material The online version of this article (doi:10.1007/s12015-016-9662-8) contains supplementary material, which is available to authorized users. |
Databáze: | OpenAIRE |
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