Evidence for the existence of two forms of RNA polymerases I and II in insect wing epidermis
| Autor: | S. Sridhara, Lawrence I. Gilbert, J. Nowock |
|---|---|
| Rok vydání: | 1978 |
| Předmět: |
Amanitins
RNA-dependent RNA polymerase Antheraea pernyi Moths Biochemistry Genetics and Molecular Biology (miscellaneous) Divalent RNA Polymerase I Transcription (biology) RNA polymerase I Animals Magnesium Polymerase Skin chemistry.chemical_classification Manganese biology RNA DNA-Directed RNA Polymerases biology.organism_classification Molecular biology Isoenzymes Lepidoptera Kinetics chemistry Biochemistry biology.protein RNA Polymerase II Small nuclear RNA |
| Zdroj: | Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis. 520:393-403 |
| ISSN: | 0005-2787 |
| DOI: | 10.1016/0005-2787(78)90236-8 |
| Popis: | DNA-dependent RNA polymerases were solubilized from developing wings of the oak silkmoth, Antheraea pernyi, and partially purified by ion-exchange chromatography and sucrose gradient sedimentation. Four enzyme species were resolved on the basis of chromatographic behavior, divalent cation requirements, ionic strength optima, template preference and alpha-amanitin sensitivity. Each class (i.e. RNA polymerase I and II) was present in two forms termed IA, IB and IIA, IIB on the basis of their elution pattern from the column. Both class I enzymes were sensitive to high concentrations of alpha-amanitin but this may be due to general toxicity rather than specific inhibition. The intraclass variants did not differ significantly in enzymatic properties although form IIB was more sensitive to alpha-amanitin (50% inhibition at 2 . 10(-9) M) than form IIA (3 . 10(-8)M). |
| Databáze: | OpenAIRE |
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