Crystallization and preliminary crystallographic studies of bar-headed goose fluoromethaemoglobin with inositol hexaphosphate
| Autor: | Guang-Ying Lu, Jia-Peng Zhu, Yu-He Liang, Huan-Chen Wang |
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| Rok vydání: | 2000 |
| Předmět: |
Phytic Acid
Cooperativity Crystallography X-Ray law.invention Crystal Hemoglobins Goose Allosteric Regulation Structural Biology law biology.animal Geese β subunit PEG ratio Animals Inositol hexaphosphate Crystallization Methemoglobin Binding Sites biology Chemistry General Medicine Oxygen Crystallography Protein Binding Bar (unit) |
| Zdroj: | Acta Crystallographica Section D Biological Crystallography. 56:1183-1184 |
| ISSN: | 0907-4449 |
| DOI: | 10.1107/s0907444900008660 |
| Popis: | Bar-headed goose fluoromethaemoglobin (fluoromet-Hb) complexed with inositol hexaphosphate (IHP) has been crystallized using PEG 6000 as precipitant. The crystal belongs to space group P2(1), with unit-cell parameters a = 59.8, b = 72.0, c = 79.8 A, beta = 102.1 degrees, and diffracts to 2.5 A resolution. To prove the presence of IHP, the structure was determined by the molecular-replacement method. IHP was observed at the entrance to the central cavity between the N and C termini of two beta subunits. |
| Databáze: | OpenAIRE |
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