In Vitro Transport of Active α1-Antitrypsin to the Apical Surface of Epithelia by Targeting the Polymeric Immunoglobulin Receptor
| Autor: | Catherine L. Silski, Elizabeth Eckman, Tivadar Szegletes, Pamela B. Davis, John R. Schreiber, William D. Mallender, Thomas W. Ferkol |
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| Rok vydání: | 1999 |
| Předmět: |
Pulmonary and Respiratory Medicine
Recombinant Fusion Proteins Clinical Biochemistry Transfection Cell Line Proinflammatory cytokine Mice medicine Animals Humans Receptor Immunoglobulin Fragments Molecular Biology Dose-Response Relationship Drug biology Elastase Receptors Polymeric Immunoglobulin Biological Transport Epithelial Cells Cell Biology Fusion protein Epithelium Cell biology Kinetics medicine.anatomical_structure Biochemistry alpha 1-Antitrypsin Neutrophil elastase biology.protein Respiratory epithelium Polymeric immunoglobulin receptor |
| Zdroj: | American Journal of Respiratory Cell and Molecular Biology. 21:246-252 |
| ISSN: | 1535-4989 1044-1549 |
| DOI: | 10.1165/ajrcmb.21.2.3687 |
| Popis: | In cystic fibrosis (CF), the intense host inflammatory response to chronic infection largely accounts for the progressive pulmonary disease, and ultimately death. Neutrophils are the prominent inflammatory cells in the lungs of patients with CF, and large amounts of neutrophil elastase (NE) are released during phagocytosis. Besides having direct effects on structural elastin, NE stimulates the release of proinflammatory mediators from the respiratory epithelium and is a potent secretogogue. Therapeutic use of elastase inhibitors in CF has been complicated by difficulties in delivery to the critical site in the airway-the surface of the epithelium. We describe a unique strategy to protect the respiratory epithelial cell surface directly by capitalizing on the nondegradative transcytotic pathway of the polymeric immunoglobulin receptor (pIgR). A recombinant fusion protein was constructed consisting of an antihuman pIgR single-chain Fv (scFv) antibody linked to human alpha(1)-antitrypsin (A1AT), an inhibitor of NE. The recombinant scFv-A1AT fusion protein bound specifically to the pIgR on the basolateral surface of an epithelial cell monolayer, and was transported and released into the apical medium where the A1AT domain was capable of forming an inactivation complex with NE. Thus, A1AT linked to an antihuman pIgR scFv was delivered in receptor-specific fashion from the basolateral to apical surface and was released as an active antiprotease, indicating that it is feasible to deliver therapeutic proteins to the apical surface of epithelia by targeting the pIgR. |
| Databáze: | OpenAIRE |
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