Scale-Up Preparation of Crocins I and II from Gardeniajasminoides by a Two-Step Chromatographic Approach and Their Inhibitory Activity Against ATP Citrate Lyase
| Autor: | Wenbo Yu, Honghong Wu, Yinan Liu, Fengrui Song, Zifeng Pi, Jing-Ya Li, Qiaoli Pu, Shuguang Guan, Shu Liu, De-An Guo |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
inhibitory activity on ATP citrate lyase
ATP citrate lyase macroporous resin column chromatography Two step Pharmaceutical Science Organic chemistry Gardenia jasminoides crocins Analytical Chemistry Preparation method 03 medical and health sciences 0302 clinical medicine Column chromatography QD241-441 Drug Discovery Ic50 values Physical and Theoretical Chemistry high-speed counter-current chromatography 030304 developmental biology 0303 health sciences Chromatography biology Chemistry biology.organism_classification Solvent Chemistry (miscellaneous) 030220 oncology & carcinogenesis SCALE-UP Molecular Medicine preparation method |
| Zdroj: | Molecules, Vol 26, Iss 3137, p 3137 (2021) Molecules Volume 26 Issue 11 |
| ISSN: | 1420-3049 |
| Popis: | Crocins are highly valuable natural compounds for treating human disorders, and they are also high-end spices and colorants in the food industry. Due to the limitation of obtaining this type of highly polar compound, the commercial prices of crocins I and II are expensive. In this study, macroporous resin column chromatography combined with high-speed counter-current chromatography (HSCCC) was used to purify crocins I and II from natural sources. With only two chromatographic steps, both compounds were simultaneously isolated from the dry fruit of Gardenia jasminoides, which is a cheap herbal medicine distributed in a number of countries. In an effort to shorten the isolation time and reduce solvent usage, forward and reverse rotations were successively utilized in the HSCCC isolation procedure. Crocins I and II were simultaneously obtained from a herbal resource with high recoveries of 0.5% and 0.1%, respectively, and high purities of 98.7% and 99.1%, respectively, by HPLC analysis. The optimized preparation method was proven to be highly efficient, convenient, and cost-effective. Crocins I and II exhibited inhibitory activity against ATP citrate lyase, and their IC50 values were determined to be 36.3 ± 6.24 and 29.7 ± 7.41 μM, respectively. |
| Databáze: | OpenAIRE |
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