In Vitro Culture of Chicken Circulating and Gonadal Primordial Germ Cells on a Somatic Feeder Layer of Avian Origin
| Autor: | Michelle Paradowska, Takashi Kuwana, Marek Bednarczyk, Agata Szczerba |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
endocrine system
Somatic cell chicken Cell Basic fibroblast growth factor cPGCs embryo Biology Article 03 medical and health sciences chemistry.chemical_compound Feeder Layer Lipid droplet lcsh:Zoology medicine primordial germ cells lcsh:QL1-991 feeder cell layer 030304 developmental biology 0303 health sciences lcsh:Veterinary medicine General Veterinary urogenital system fungi 0402 animal and dairy science Embryo 04 agricultural and veterinary sciences 040201 dairy & animal science In vitro Cell biology medicine.anatomical_structure chemistry Cytoplasm embryonic structures lcsh:SF600-1100 Animal Science and Zoology gPGCs |
| Zdroj: | Animals Volume 10 Issue 10 Animals : an Open Access Journal from MDPI Animals, Vol 10, Iss 1769, p 1769 (2020) |
| ISSN: | 2076-2615 |
| DOI: | 10.3390/ani10101769 |
| Popis: | The present study had two aims: (1) To develop a culture system that imitates a normal physiological environment of primordial germ cells (PGCs). There are two types of PGCs in chicken: Circulating blood (cPGCs) and gonadal (gPGCs). The culture condition must support the proliferation of both cPGCs and gPGCs, without affecting their migratory properties and must be deprived of xenobiotic factors, and (2) to propose an easy-to-train, nonlabeling optical technique for the routine identification of live PGCs. To address the first aim, early chicken embryo&rsquo s feeder cells were examined instead of using feeder cells from mammalian species. The KAv-1 medium at pH 8.0 with the addition of bFGF (basic fibroblast growth factor) was used instead of a conventional culture medium (pH approximately 7.2). Both cPGCs and gPGCs proliferated in vitro and retained their migratory ability after 2 weeks of culture. The cultivated cPGCs and gPGCs colonized the right and/or left gonads of the recipient male and female embryos. To address the second aim, we demonstrated a simple and rapid method to identify live PGCs as bright cells under darkfield illumination. The PGCs rich in lipid droplets in their cytoplasm highly contrasted with the co-cultured feeder layer and other cell populations in the culture. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
| Nepřihlášeným uživatelům se plný text nezobrazuje | K zobrazení výsledku je třeba se přihlásit. |