Comparative studies of immobilized lipase- and acid-catalyzed fatty acid methyl ester synthesis for seed lipid analysis
| Autor: | Young Soo Keum, Chang-Soo Kim, Si-Kyung Lee |
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| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
chemistry.chemical_classification biology 010401 analytical chemistry Triacylglycerol lipase Fatty acid Transesterification 01 natural sciences Applied Microbiology and Biotechnology Toluene Article 0104 chemical sciences chemistry.chemical_compound chemistry 010608 biotechnology biology.protein Degradation (geology) Organic chemistry Lipase Isomerization Fatty acid methyl ester Food Science Biotechnology |
| Zdroj: | Food Science and Biotechnology. 25:771-776 |
| ISSN: | 2092-6456 1226-7708 |
| Popis: | Fatty acids are one of the most important nutrients in food. Acid- or base-catalyzed transesterification methods are commonly used for the analysis of fatty acids. However, several drawbacks were reported for these methods, including the isomerization and degradation of fatty acids. Lipase-catalyzed reactions are usually undertaken at mild conditions, preventing such problems. In this study, commercial resin-bound lipase from Candida antartica was tested for possible application in fatty acid methyl ester analysis. Experimental parameters, including temperature, reaction time, and re-cycling were evaluated. The optimized condition was (5-10 mg lipid, 0.5 mL of MeOH, and 50 mg Novozyme 435 in 2 mL toluene, 80°C for 1 h). In optimized condition, the lipase-catalyzed methods yielded similar results with the chemical method. In overall, lipase-catalyzed transesterification can be a useful alternative to acid-catalyzed methods for fatty acid analysis. |
| Databáze: | OpenAIRE |
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