The safety of β-carotene from Yarrowia lipolytica
| Autor: | Daniel Grenfell-Lee, Renato Cardoso, Kresimir Pucaj, Samuel Zeller |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
medicine.medical_treatment
Yarrowia CHO Cells Pharmacology Toxicology medicine.disease_cause Ames test Rats Sprague-Dawley Cricetulus Cricetinae medicine Animals Dose-Response Relationship Drug biology Mutagenicity Tests Chemistry Chinese hamster ovary cell Toxicity Tests Subchronic Carotene General Medicine Plants Genetically Modified beta Carotene biology.organism_classification Rats Genetically modified organism Toxicity Micronucleus test Genotoxicity Food Science |
| Zdroj: | Food and Chemical Toxicology. 65:1-11 |
| ISSN: | 0278-6915 |
| DOI: | 10.1016/j.fct.2013.12.010 |
| Popis: | Crystalline β-carotene from genetically modified Yarrowia lipolytica is an alternative source of β-carotene for use as a nutritional supplement. To support the use of β-carotene from Y. lipolytica as a food ingredient, the genotoxic and subchronic toxicity potential of this compound was determined. Genotoxicity was examined using Salmonella typhimurium and Escherichia coli (Ames test), a chromosomal aberration assay in Chinese Hamster Ovary WBL cells, and the micronucleus test in CD-1 mice. All three assays showed no significant results due to β-carotene from Y. lipolytica. In a subchronic toxicity study in SD rats, β-carotene from Y. lipolytica was administered by oral gavage for 13weeks at 0, 125, 250 or 500mg/kg per day. Adverse effects were not observed following clinical, clinical pathology and gross- and histopathological evaluations of dosed rats; thus, the no-observed-adverse effect level (NOAEL) for β-carotene from Y. lipolytica was 500mg/kg, the highest dose used in the study. In conclusion, β-carotene derived from Y. lipolytica was shown in genotoxicity models and a standard rat subchronic rat study to have a safety profile similar to that of the current commercial products (synthetic and natural) with no unexpected finding attributable to the alternative source. |
| Databáze: | OpenAIRE |
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