Glucose and Lipid Dysmetabolism in a Rat Model of Prediabetes Induced by a High-Sucrose Diet

Autor: Frederico C. Pereira, Ana Burgeiro, Paula Neto, Eugenia Carvalho, Manuela G. Cerqueira, Flávio Reis, Bárbara María Varela-Rodríguez, Sara Nunes
Předmět:
Blood Glucose
Male
Glucose uptake
medicine.medical_treatment
high-sucrose diet
prediabetes
glucose
lipid
metabolism
hypertriglyceridemia
030204 cardiovascular system & hematology
0302 clinical medicine
Dietary Sucrose
Insulin
Prediabetes
Adiposity
Glucose Transporter Type 2
Hypertriglyceridemia
Glucose Transporter Type 1
Nutrition and Dietetics
Fasting
Lipid
3. Good health
Postprandial
Liver
Lipotoxicity
Glucose-6-Phosphatase
medicine.medical_specialty
High-sucrose diet
030209 endocrinology & metabolism
Biology
Article
Prediabetic State
03 medical and health sciences
Internal medicine
Lipid biosynthesis
medicine
Animals
Lipolysis
Rats
Wistar
Muscle
Skeletal
Triglycerides
Lipid metabolism
Lipid Metabolism
medicine.disease
Rats
Disease Models
Animal
Endocrinology
Glucose
Metabolism
Diabetes Mellitus
Type 2
Insulin Resistance
Food Science
Zdroj: CIÊNCIAVITAE
RUC. Repositorio da Universidade da Coruña
instname
Nutrients; Volume 9; Issue 6; Pages: 638
Nutrients
DOI: 10.3390/nu9060638
Popis: [Abstract] Glucotoxicity and lipotoxicity are key features of type 2 diabetes mellitus, but their molecular nature during the early stages of the disease remains to be elucidated. We aimed to characterize glucose and lipid metabolism in insulin-target organs (liver, skeletal muscle, and white adipose tissue) in a rat model treated with a high-sucrose (HSu) diet. Two groups of 16-week-old male Wistar rats underwent a 9-week protocol: HSu diet (n = 10)—received 35% of sucrose in drinking water; Control (n = 12)—received vehicle (water). Body weight, food, and beverage consumption were monitored and glucose, insulin, and lipid profiles were measured. Serum and liver triglyceride concentrations, as well as the expression of genes and proteins involved in lipid biosynthesis were assessed. The insulin-stimulated glucose uptake and isoproterenol-stimulated lipolysis were also measured in freshly isolated adipocytes. Even in the absence of obesity, this rat model already presented the main features of prediabetes, with fasting normoglycemia but reduced glucose tolerance, postprandial hyperglycemia, compensatory hyperinsulinemia, as well as decreased insulin sensitivity (resistance) and hypertriglyceridemia. In addition, impaired hepatic function, including altered gluconeogenic and lipogenic pathways, as well as increased expression of acetyl-coenzyme A carboxylase 1 and fatty acid synthase in the liver, were observed, suggesting that liver glucose and lipid dysmetabolism may play a major role at this stage of the disease. Portugal. Fundação para a Ciência e a Tecnologia; FCT; EXCL/DTP-PIC/0069/2012 Portugal. Fundação para a Ciência e a Tecnologia; FCT; UID/NEU/04539/2013 European Commision; FCOMP-01-0124-FEDER-028417 European Commision; POCI-01-0145-FEDER-007440 European Commision; CENTRO-01-0145-FEDER-000012 European Commision; CENTRO-01-0145-FEDER-000008
Databáze: OpenAIRE
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