Pilot for Validation of Online Pretreatments for Analyses of Organics by Gas Chromatography-Mass Spectrometry: Application to Space Research
| Autor: | J. Labanowski, Claude Geffroy-Rodier, Marc David, N.-Y. Musadji, Robert Sternberg |
|---|---|
| Přispěvatelé: | Institut de Chimie des Milieux et Matériaux de Poitiers (IC2MP), Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université de Poitiers-Institut de Chimie du CNRS (INC) |
| Rok vydání: | 2016 |
| Předmět: |
chemistry.chemical_classification
Tetramethylammonium hydroxide Chromatography Silylation Biomolecule 010401 analytical chemistry Mars Exploration Program Mass spectrometry 01 natural sciences 0104 chemical sciences Analytical Chemistry chemistry.chemical_compound chemistry 13. Climate action Reagent 0103 physical sciences [CHIM]Chemical Sciences Gas chromatography–mass spectrometry Derivatization 010303 astronomy & astrophysics |
| Zdroj: | Analytical Chemistry Analytical Chemistry, American Chemical Society, 2016, ⟨10.1021/acs.analchem.6b00052⟩ |
| ISSN: | 1520-6882 0003-2700 |
| DOI: | 10.1021/acs.analchem.6b00052⟩ |
| Popis: | International audience; The search for complex organic molecules in extraterrestrial environments, including important biomolecules such as amino and fatty acids, will require a space compatible sample handling system to enable their detection by gas chromatography-mass spectrometry (GC-MS). For the future Mars exploratory mission Exomars 2018 aimed at organic molecules detection, a dedicated laboratory pilot, called Device for Pretreatment of Sample (DPS), reproducing representative space operating conditions has been developed. After its optimization, it aimed at validating under development protocols and interpreting forthcoming in situ resulting data. The DPS, dedicated to organic compounds' analysis, is discussed in terms of its technical features. The derivatization is carried out on a 50-100 mg mineral sample in a 4 mL reactor coupled with a GC-MS injector to simulate on line in situ derivatization-volatilization-transfer steps. Three derivatization reactions have been carried out with N-methyl-N-(tert-butyldimethylsilyl)-trifluoroacetamide (MTBSTFA) as silylating reagent, N,N-dimethylformamide dimethylacetal (DMF-DMA) and tetramethylammonium hydroxide (TMAH) as methylating agents. The performances are illustrated by comparison of conventional and in situ silylation, developed for space research applications, using terrestrial mineral matrix and Mars analog materials enriched with 25 nmol of each targeted organic molecule. The work presented in this rationale has established that the use of derivatization reactions widens the scope of targeted molecules but also clearly points out mineral matrix effect. Decreasing mineral influence on pretreatment will be the next scientific challenge in in situ analysis. |
| Databáze: | OpenAIRE |
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