The expression and prognostic value of stem cell markers Bmi-1, HESC5:3, and HES77 in human papillomavirus–positive and –negative oropharyngeal squamous cell carcinoma
| Autor: | Lauri Jouhi, Timo Atula, Hesham Mohamed, Antti Mäkitie, Jaana Hagström, Caj Haglund, Alhadi Almangush |
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| Přispěvatelé: | Department of Pathology, University of Helsinki, HUSLAB, HUS Head and Neck Center, Medicum, CAN-PRO - Translational Cancer Medicine Program, Research Programs Unit, Faculty of Medicine, Korva-, nenä- ja kurkkutautien klinikka, Clinicum, Department of Ophthalmology and Otorhinolaryngology, Research Program in Systems Oncology, HUS Abdominal Center, Department of Surgery, II kirurgian klinikka |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Human Papillomavirus Positive Epithelial-Mesenchymal Transition 3122 Cancers Cell p16 Stem cell marker Carcinoma Squamous Cell/*pathology/virology Oropharyngeal Neoplasms/*pathology/*virology 03 medical and health sciences 0302 clinical medicine Epithelial-Mesenchymal Transition/genetics Polycomb Repressive Complex 1/*biosynthesis medicine Humans human papillomavirus Papillomaviridae Antibodies Monoclonal/immunology RC254-282 Cancer Cyclin-Dependent Kinase Inhibitor Proteins Polycomb Repressive Complex 1 Tissue microarray Oncogene business.industry Papillomavirus Infections Cyclin-Dependent Kinase Inhibitor Proteins/*biosynthesis/genetics Antibodies Monoclonal Neoplasms. Tumors. Oncology. Including cancer and carcinogens General Medicine 3126 Surgery anesthesiology intensive care radiology medicine.disease Embryonic stem cell Papillomavirus Infections/virology 3. Good health Oropharyngeal Neoplasms 030104 developmental biology medicine.anatomical_structure 030220 oncology & carcinogenesis immunohistochemistry Papillomaviridae/*isolation & purification Carcinoma Squamous Cell Cancer research Immunohistochemistry oropharynx 3111 Biomedicine business |
| Zdroj: | Tumor Biology, Vol 41 (2019) |
| ISSN: | 1423-0380 1010-4283 |
| DOI: | 10.1177/1010428319840473 |
| Popis: | Human papillomavirus is detected in over 50% of oropharyngeal squamous cell carcinomas. Human papillomavirus–positive oropharyngeal squamous cell carcinomas differ from human papillomavirus–negative tumors, and both expression patterns are classified as distinct entities. The Bmi-1 oncogene is a well-known member of the mammalian polycomb-group family. HESC5:3 and HES77 are newly developed monoclonal antibodies produced against undifferentiated embryonic stem cells. Our aim was to explore their roles in both human papillomavirus–positive and –negative oropharyngeal squamous cell carcinomas. Our cohort comprised 202 consecutive oropharyngeal squamous cell carcinoma patients diagnosed and treated with curative intent. We used tissue microarray tumor blocks to study the immunohistochemical expression of Bmi-1, HESC5:3, and HES77. We compared the expressions of these stem cell markers with p16 immunoexpression and human papillomavirus status, as well as with other characteristics of the tumor, and with patients’ clinical data and follow-up data. Human papillomavirus– and p16-positive tumors expressed less Bmi-1 and more HESC5:3 than the negative tumors. HES77 expression was high in human papillomavirus–positive oropharyngeal squamous cell carcinoma, but it did not correlate with p16 positivity. In our multivariable model, Bmi-1 and HESC5:3 were still associated with human papillomavirus, but the association between human papillomavirus and HES77 remained absent. In conclusion, Bmi-1, HESC5:3, and HES77 may have a different role in human papillomavirus–positive and human papillomavirus–negative tumors. There was no correlation between Bmi-1, HESC5:3, and HES77 expression and survival. |
| Databáze: | OpenAIRE |
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