Expression of epidermal CAMP changes in parallel with permeability barrier status

Autor: Soyun Cho, Melanie Hupe, Walter M. Holleran, Gemma Martin-Ezquerra, Carles Trullas, Jong-Kyung Youm, Peter M. Elias, Donald S. Mackenzie, Mao-Qiang Man, Marina Rodríguez-Martín, Katherine A. Radek
Rok vydání: 2011
Předmět:
Male
Aging
beta-Defensins
Cell Membrane Permeability
medicine.medical_treatment
calcipotriol
vitamin D
Lamellar granule
Hairless
Biochemistry
PPAR
Cathelicidin
030207 dermatology & venereal diseases
Mice
0302 clinical medicine
Models
cathelicidin
Barrier function
Mice
Knockout

0303 health sciences
catestatin
beta-defensin
Cell biology
Models
Animal

Antimicrobial peptides
LXR
Female
Ultraviolet Rays
Knockout
Clinical Sciences
Oncology and Carcinogenesis
Dermatology
urea
Biology
Stress
Article
03 medical and health sciences
Cathelicidins
barrier repair
medicine
Animals
Molecular Biology
psychological stress
030304 developmental biology
permeability barrier
Mice
Hairless

Epidermis (botany)
Animal
Dermatology & Venereal Diseases
Cell Biology
Peptide Fragments
imiquimod
Beta defensin
Permeability (electromagnetism)
Psychological
Chromogranin A
Epidermis
Immunostaining
Stress
Psychological

Antimicrobial Cationic Peptides
Zdroj: The Journal of investigative dermatology, vol 131, iss 11
The Journal of investigative dermatology
Popis: Two critical defensive functions of the outer epidermis, the permeability barrier and antimicrobial defense, share certain structural and biochemical features. Moreover, three antimicrobial peptides (AMPs), i.e., mouse β-defensin 3 (mBD3), mouse cathelicidin antimicrobial peptide (mCAMP), and the neuroendocrine peptide, catestatin (Cst), all localize to the outer epidermis, and both mBD3 and mCAMP are secreted from the epidermal lamellar bodies with other organelle contents that subserve the permeability barrier. These three AMPs are upregulated in response to acute permeability barrier disruption, whereas conversely, mCAMP-/- mice (unable to combat Gram-positive pathogens) also display abnormal barrier homeostasis. To determine further whether these two functions are co-regulated, we investigated changes in immunostaining for these three AMPs in skin samples in which the permeability barrier function in mice had been either compromised or enhanced. Compromised or enhanced barrier function correlated with reduced or enhanced immunohistochemical expression of mCAMP, respectively, but conversely with Cst expression, likely due to the role of this AMP as an endogenous inhibitor of cathelicidin expression. mBD3 expression correlated with experimental barrier perturbations, but poorly with developmental changes in barrier function. These studies show that changes in cathelicidin and Cst expression parallel changes in permeability barrier status, with a less clear relationship with mBD3 expression.
Databáze: OpenAIRE