Expression of epidermal CAMP changes in parallel with permeability barrier status
Autor: | Soyun Cho, Melanie Hupe, Walter M. Holleran, Gemma Martin-Ezquerra, Carles Trullas, Jong-Kyung Youm, Peter M. Elias, Donald S. Mackenzie, Mao-Qiang Man, Marina Rodríguez-Martín, Katherine A. Radek |
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Rok vydání: | 2011 |
Předmět: |
Male
Aging beta-Defensins Cell Membrane Permeability medicine.medical_treatment calcipotriol vitamin D Lamellar granule Hairless Biochemistry PPAR Cathelicidin 030207 dermatology & venereal diseases Mice 0302 clinical medicine Models cathelicidin Barrier function Mice Knockout 0303 health sciences catestatin beta-defensin Cell biology Models Animal Antimicrobial peptides LXR Female Ultraviolet Rays Knockout Clinical Sciences Oncology and Carcinogenesis Dermatology urea Biology Stress Article 03 medical and health sciences Cathelicidins barrier repair medicine Animals Molecular Biology psychological stress 030304 developmental biology permeability barrier Mice Hairless Epidermis (botany) Animal Dermatology & Venereal Diseases Cell Biology Peptide Fragments imiquimod Beta defensin Permeability (electromagnetism) Psychological Chromogranin A Epidermis Immunostaining Stress Psychological Antimicrobial Cationic Peptides |
Zdroj: | The Journal of investigative dermatology, vol 131, iss 11 The Journal of investigative dermatology |
Popis: | Two critical defensive functions of the outer epidermis, the permeability barrier and antimicrobial defense, share certain structural and biochemical features. Moreover, three antimicrobial peptides (AMPs), i.e., mouse β-defensin 3 (mBD3), mouse cathelicidin antimicrobial peptide (mCAMP), and the neuroendocrine peptide, catestatin (Cst), all localize to the outer epidermis, and both mBD3 and mCAMP are secreted from the epidermal lamellar bodies with other organelle contents that subserve the permeability barrier. These three AMPs are upregulated in response to acute permeability barrier disruption, whereas conversely, mCAMP-/- mice (unable to combat Gram-positive pathogens) also display abnormal barrier homeostasis. To determine further whether these two functions are co-regulated, we investigated changes in immunostaining for these three AMPs in skin samples in which the permeability barrier function in mice had been either compromised or enhanced. Compromised or enhanced barrier function correlated with reduced or enhanced immunohistochemical expression of mCAMP, respectively, but conversely with Cst expression, likely due to the role of this AMP as an endogenous inhibitor of cathelicidin expression. mBD3 expression correlated with experimental barrier perturbations, but poorly with developmental changes in barrier function. These studies show that changes in cathelicidin and Cst expression parallel changes in permeability barrier status, with a less clear relationship with mBD3 expression. |
Databáze: | OpenAIRE |
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