Targeted hexagonal Pd nanosheet combination therapy for rheumatoid arthritis via the photothermal controlled release of MTX
| Autor: | Yayu Wen, Taoyuan Xu, Mengmeng Xu, Jie Liu, Xu Chen, Xufeng Zhu, Xiuying Qin, Yanan Liu |
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| Rok vydání: | 2019 |
| Předmět: |
musculoskeletal diseases
Combination therapy Biomedical Engineering Arthritis 02 engineering and technology Pharmacology 010402 general chemistry 01 natural sciences Arthritis Rheumatoid Mice In vivo Human Umbilical Vein Endothelial Cells medicine Animals Humans General Materials Science skin and connective tissue diseases Chemistry General Chemistry General Medicine Photothermal therapy 021001 nanoscience & nanotechnology medicine.disease Controlled release Nanostructures 0104 chemical sciences HEK293 Cells Methotrexate RAW 264.7 Cells Photochemotherapy Mice Inbred DBA Antirheumatic Agents Delayed-Action Preparations Rheumatoid arthritis Toxicity Female 0210 nano-technology Palladium medicine.drug |
| Zdroj: | Journal of Materials Chemistry B. 7:112-122 |
| ISSN: | 2050-7518 2050-750X |
| DOI: | 10.1039/c8tb02302f |
| Popis: | Methotrexate (MTX) is a drug that is used for the clinical treatment of rheumatoid arthritis (RA), a stubborn disease caused by over-immunization. However, the toxicity that arises as a result of poor selectivity to inflammatory cells severely limits the application of MTX. Therefore, new therapeutic strategies are needed for treating RA. Here, we describe the design and synthesis of a nanotherapy agent, Pd-Cys@MTX@RGD, which can target inflammatory cells and control MTX release. The novel hexagonal palladium (Pd) nanosheets were used as a near-infrared (NIR) photothermal agent modified with arginine-glycineaspartic acid (RGD) peptides on the surface to enhance the ability of the nanosheet targeting of inflammatory cells. In subsequent experiments, the Pd-Cys@MTX@RGD nanosheets were observed to greatly reduce the toxicity of MTX, showing controlled MTX release under irradiation of 808 nm (0.3 W cm-2). Moreover, taking advantage of the fact that MTX can be combined with multiple therapeutic methods, the photothermal therapy (PTT) of Pd nanosheets provided a compensatory effect to enhance the therapeutic efficacy of MTX. Under combination therapy, Pd-Cys@MTX@RGD was shown to effectively inhibit the inflammatory response induced by vascular endothelial growth factor (VEGF) and IL-1β. And, in vivo, multifunctional Pd-Cys@MTX@RGD effectively inhibited the symptoms of RA by inhibiting the expression of pro-inflammatory cytokines (TNF-α,COX-2). We hope that the construction of nanomaterials can add potential value to the design of chemical drugs and therapeutic strategies for RA. |
| Databáze: | OpenAIRE |
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