Gas chromatographic quadrupole time-of-flight full scan high resolution mass spectrometric screening of human urine in antidoping analysis

Autor: Wadha Abushareeda, Sameera Alyazidi, Suhail Kraiem, Emmanouil Lyris, Najib Dbes, Arjen Lommen, Costas Georgakopoulos, Michel W. F. Nielen, Mohammed Alsayrafi, Peter Horvatovich, Aisha Al Wahaibi
Přispěvatelé: Analytical Biochemistry, Medicinal Chemistry and Bioanalysis (MCB)
Jazyk: angličtina
Rok vydání: 2017
Předmět:
Monsteradministratie & Coördinatie
Clinical Biochemistry
Tandem mass spectrometry
01 natural sciences
Biochemistry
Analytical Chemistry
human experiment
chemistry.chemical_compound
BU Contaminants & Toxins
Anabolic Agents
conjugate
Limit of Detection
derivatization
enzymatic degradation
Human urine
Child
Doping in Sports
Gas chromatography
quantitative analysis
Chemistry
Epitestosterone
General Medicine
time of flight mass spectrometry
Organische Chemie
hydrolysis
Female
Electronic data
athlete
Full scan high resolution
trimethylsilyl derivative
metabolite
BU Contaminanten & Toxines
doping
Androsterone
Mass spectrometry
Gas Chromatography-Mass Spectrometry
remission
tandem mass spectrometry
Humans
liquid chromatography
controlled study
diagnostic test accuracy study
human
Derivatization
VLAG
Detection limit
Chromatography
controlled clinical trial
010405 organic chemistry
screening
010401 analytical chemistry
Organic Chemistry
Reproducibility of Results
Cell Biology
human tissue
0104 chemical sciences
phase 2 clinical trial
Anabolic steroids
anabolic agent
sensitivity and specificity
validation process
designer drug
Linear Models
extraction
ion
Gas chromatography–mass spectrometry
Time-of-flight mass spectrometry
urine sampling
Zdroj: Journal of Chromatography. B, Analytical technologies in the biomedical and life sciences, 1063, 74-83
Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences, 1063, 74-83. ELSEVIER SCIENCE BV
Journal of Chromatography. B, Analytical technologies in the biomedical and life sciences 1063 (2017)
ISSN: 1570-0232
Popis: This paper presents the development and validation of a high-resolution full scan (FS) electron impact ionization (EI) gas chromatography coupled to quadrupole Time-of-Flight mass spectrometry (GC/QTOF) platform for screening anabolic androgenic steroids (AAS) in human urine samples. The World Antidoping Agency (WADA) enlists AAS as prohibited doping agents in sports, and our method has been developed to comply with the qualitative specifications of WADA to be applied for the detection of sports antidoping prohibited substances, mainly for AAS. The method also comprises of the quantitative analysis of the WADA's Athlete Biological Passport (ABP) endogenous steroidal parameters. The applied preparation of urine samples includes enzymatic hydrolysis for the cleavage of the Phase II glucuronide conjugates, generic liquid liquid extraction and trimethylsilyl (TMS) derivatization steps. Tandem mass spectrometry (MS/MS) acquisition was applied on few selected ions to enhance the specificity and sensitivity of GC/TOF signal of few compounds. The full scan high resolution acquisition of analytical signal, for known and unknown TMS derivatives of AAS provides the anti doping system with a new analytical tool for the detection designer drugs and novel metabolites, which prolongs the AAS detection, after electronic data files' reprocessing. The current method is complementary to the respective liquid chromatography coupled to mass spectrometry (LC/MS) methodology widely used to detect prohibited molecules in sport, which cannot be efficiently ionized with atmospheric pressure ionization interface.
Databáze: OpenAIRE
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