rhBMP-2 Modulation of Gene Expression in Infected Segmental Bone Defects
| Autor: | Jamie L. Lohr, William D. Lew, Xinqian Chen, Louis S. Kidder, Andrew H. Schmidt, Katherine E. Brick |
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| Rok vydání: | 2008 |
| Předmět: |
Surgical Sponges
Staphylococcus aureus Time Factors Osteocalcin Bone Morphogenetic Protein 2 Bone healing Bone Morphogenetic Protein Receptors Type II Bone morphogenetic protein 2 Collagen Type I Bone remodeling Rats Sprague-Dawley Andrology Gene expression Animals Humans Orthopedics and Sports Medicine Femur RNA Messenger Receptor Collagen Type II Fracture Healing Drug Carriers Messenger RNA biology General Medicine Staphylococcal Infections Bone Diseases Infectious Recombinant Proteins Rats Up-Regulation Disease Models Animal Real-time polymerase chain reaction Immunology biology.protein Symposium: Tribute to Dr. Marshall Urist: Musculoskeletal Growth Factors Surgery Bone Remodeling Femoral Fractures |
| Zdroj: | Clinical Orthopaedics and Related Research®. 467:3096-3103 |
| ISSN: | 1528-1132 0009-921X |
| DOI: | 10.1007/s11999-008-0599-3 |
| Popis: | The osteoinductive capability of BMPs appears diminished in the setting of acute infection. We applied rhBMP-2 to a segmental defect in a rat femur and measured the expression of key bone formation genes in the presence of acute infection. Types I and II collagen, osteocalcin, and BMP Type II receptor mRNA expression were characterized in 72 Sprague-Dawley rats, which received either bovine collagen carrier with 200 mug rhBMP-2 plus Staphylococcus aureus, carrier with bacteria only, carrier with rhBMP-2 only, or carrier alone. Six animals from each group were euthanized at 1, 2, and 4 weeks. Total RNA was isolated and extracted, and mRNA was determined by real-time comparative quantitative PCR. Infected defects had little expression of collagen I and II and osteocalcin mRNAs, while BMP receptor II expression with infection was greater than carrier-only controls at weeks 2 and 4. Notably, all four genes were upregulated in infected defects in the presence of rhBMP-2. Thus, in a clinical setting with a high risk of infection and nonunion, such as a compound fracture with bone loss, rhBMP-2 may increase the rate and extent of bone formation. Even if infection does occur, rhBMP-2 may allow a quicker overall recovery time. |
| Databáze: | OpenAIRE |
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