The H+/ATP coupling ratio of the ATP synthase from thiol-modulated chloroplasts and two cyanobacterial strains is four
Autor: | Hendrika S. Van Walraven, Heinrich Strotmann, Bernd Rumberg, Oliver Schwarz |
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Jazyk: | angličtina |
Předmět: |
Chloroplasts
Stereochemistry Biophysics Energy transduction Photophosphorylation Cyanobacteria Biochemistry Structural Biology ATP hydrolysis Genetics Electrochemical gradient P/O ratio Molecular Biology Enzyme regulation biology ATP synthase Chemiosmosis ATP synthase H+ Cell Biology Proton translocation Chloroplast Proton gradient Proton-Translocating ATPases H+/ATP ratio biology.protein Thermodynamics ATP synthase alpha/beta subunits |
Zdroj: | FEBS Letters. (3):309-313 |
ISSN: | 0014-5793 |
DOI: | 10.1016/0014-5793(95)01536-1 |
Popis: | In this paper the authors emphasise that the proton translocating ATP synthase from thiol-modulated chloroplasts and two cyanobacterial strains has a coupling ratio of 4 protons per ATP synthesised or hydrolysed. This ratio is determined by several thermodynamic studies at equilibrium between phosphate potential (ΔGp) and proton gradient ( Δ μ H + ), and is confirmed by measurement of proton flux during ATP hydrolysis. Ratios lower than 4 H+/ATP that have been published in the past have predominantly been determined with the oxidised chloroplast enzyme. Errors in these measurements will be discussed. |
Databáze: | OpenAIRE |
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