Luteinization of porcine preovulatory follicles leads to systematic changes in follicular gene expression
| Autor: | Cansu Agca, Gordon K. Springer, Lawrence J. Forrester, E. Antoniou, Matthew C. Lucy, Sarah J Kolath, Randall S. Prather, James E. Ries, Nagappan Mathialagan, Kristin M. Whitworth, Jae-Hwan Kim |
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| Rok vydání: | 2006 |
| Předmět: |
endocrine system
Embryology medicine.medical_specialty Microarray Swine media_common.quotation_subject Gene Expression Ovary Biology Follicle Endocrinology Ovarian Follicle Corpus Luteum Internal medicine Follicular phase Gene expression medicine Animals Ovulation In Situ Hybridization Progesterone media_common Oligonucleotide Array Sequence Analysis Estrous cycle Estradiol Reverse Transcriptase Polymerase Chain Reaction Gene Expression Profiling Obstetrics and Gynecology Cell Biology Follicular fluid Follicular Fluid Luteinization medicine.anatomical_structure Reproductive Medicine Follicular Phase Female Biomarkers |
| Zdroj: | Reproduction (Cambridge, England). 132(1) |
| ISSN: | 1470-1626 |
| Popis: | The LH surge initiates the luteinization of preovulatory follicles and causes hormonal and structural changes that ultimately lead to ovulation and the formation of corpora lutea. The objective of the study was to examine gene expression in ovarian follicles (n= 11) collected from pigs (Sus scrofa domestica) approaching estrus (estrogenic preovulatory follicle;n= 6 follicles from two sows) and in ovarian follicles collected from pigs on the second day of estrus (preovulatory follicles that were luteinized but had not ovulated;n= 5 follicles from two sows). The follicular status within each follicle was confirmed by follicular fluid analyses of estradiol and progesterone ratios. Microarrays were made from expressed sequence tags that were isolated from cDNA libraries of porcine ovary. Gene expression was measured by hybridization of fluorescently labeled cDNA (preovulatory estrogenic or -luteinized) to the microarray. Microarray analyses detected 107 and 43 genes whose expression was decreased or increased (respectively) during the transition from preovulatory estrogenic to -luteinized (P |
| Databáze: | OpenAIRE |
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