Vigabatrin in dried plasma spots: Validation of a novel LC–MS/MS method and application to clinical practice
| Autor: | Nađa Kostić, Yannis Dotsikas, Nebojša Jović, Galina Stevanović, Mirjana Medenica, Anđelija Malenović |
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| Rok vydání: | 2014 |
| Předmět: |
Analyte
Adolescent Formic acid Clinical Biochemistry Analytical chemistry Mass spectrometry Biochemistry Vigabatrin Analytical Chemistry chemistry.chemical_compound Limit of Detection Tandem Mass Spectrometry medicine Humans Child Derivatization Chromatography Chloroformates Cell Biology General Medicine Dried plasma spots chemistry Yield (chemistry) Reagent Calibration Anticonvulsants Dried Blood Spot Testing Sample collection Chromatography Liquid medicine.drug |
| Zdroj: | Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences |
| ISSN: | 1570-0232 |
| DOI: | 10.1016/j.jchromb.2014.05.037 |
| Popis: | This paper presents a LC-MS/MS method for the determination of antiepileptic drug vigabatrin in dried plasma spots (DPS). Due to its zwitterionic chemical structure, a pre-column derivatization procedure was performed, aiming to yield enhanced ionization efficiency and improved chromatographic behaviour. Propyl chloroformate, in the presence of propanol, was selected as the best derivatization reagent, providing a strong signal along with reasonable run time. A relatively novel sample collection technique, DPS, was utilized, offering easy sample handling and analysis, using a sample in micro amount (similar to 5 mu L). Derivatized vigabatrin and its internal standard, 4-aminocyclohexanecarboxylic acid, were extracted by liquid-liquid extraction (LLE) and determined in positive ion mode by applying two SRM transitions per analyte. A Zorbax Eclipse XDB-C8 column (150 x 4.6 mm, 5 mu m particle size) maintained at 30 degrees C, was utilized with running mobile phase composed of acetonitrile: 0.15% formic acid (85:15, v/v). Flow rate was 550 mu L/min and total run time 4.5 min. The assay exhibited excellent linearity over the concentration range of 0.500-50.0 mu g/mL, which is suitable for the determination of vigabatrin level after per os administration in children and youths with epilepsy, who were on vigabatrin therapy, with or without co-medication. Specificity, accuracy, precision, recovery, matrix-effect and stability were also estimated and assessed within acceptance criteria. |
| Databáze: | OpenAIRE |
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