Cytocompatibility and Bone-Formation Potential of Se-Coated 316L Stainless Steel with Nano-Pit Arrays
| Autor: | Hui Sun, Changqing Zhang, Shirong Ni, Lin Mei, Siyu Ni, Haoran Hu, Cui Ran |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Nanostructure
Materials science Biocompatibility Biomedical Engineering Pharmaceutical Science Medicine (miscellaneous) Biocompatible Materials Bioengineering 02 engineering and technology engineering.material 01 natural sciences Osseointegration Corrosion X-ray photoelectron spectroscopy Coating Osteogenesis In vivo Materials Testing 0103 physical sciences Animals General Materials Science 010304 chemical physics fungi technology industry and agriculture Stainless Steel 021001 nanoscience & nanotechnology Nanostructures Rats Chemical engineering engineering Alkaline phosphatase 0210 nano-technology |
| Zdroj: | Journal of Biomedical Nanotechnology. 14:716-724 |
| ISSN: | 1550-7033 |
| Popis: | 316L stainless steel is still widely applied in joint replacement and orthopedic surgery due to its mechanical properties, corrosion resistance and relatively low price. In this study, electrochemical oxidation and nanoscale coating were used to fabricate Se-coated 316L stainless steel with nano-pit arrays to enhance its surface characteristics, biocompatibility and osseointegration ability. The modified 316L stainless steel was tested via field emission scanning microscopy (FESEM), energy-dispersive X-ray spectrometry (EDS), X-ray photoelectron spectroscopy (XPS) and Se release studies. The results of this study showed that the nano-pit arrays were 50 nm in diameter and that the Se coating consisted primarily of elemental Se and exhibited sustained release. The biological response of the samples was evaluated using in vitro rat bone marrow mesenchymal stem cell (rBMSCs) experiments and in vivo animal experiments. The modified 316L stainless steel displays enhanced abilities of cell adhesion, proliferation and osteogenic activity, as shown by FESEM, CCK-8 assay, immunofluorescence microscopy (IF) and alkaline phosphatase (ALP) activity assay in vitro and additional new bone formation in vivo, indicating its outstanding cytocompatibility and osteogenic differentiation ability. More importantly, the Se coating can upregulate gene expression of OPN, RUNX-2 and ALP, indicating that the nano-Se-coated 316L stainless steel with nano-pit arrays is a promising biomedical material for implants in orthopedic or dental clinic applications. |
| Databáze: | OpenAIRE |
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