Ethylene promotes hyponastic growth through interaction with ROTUNDIFOLIA3/CYP90C1 in Arabidopsis
| Autor: | Laurentius A. C. J. Voesenek, Ronald Pierik, Martijn van Zanten, Danuše Tarkowská, Anton J. M. Peeters, Joanna K. Polko, Miroslav Strnad |
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| Rok vydání: | 2013 |
| Předmět: |
0106 biological sciences
Ethylene Cell division Physiology Mutant Arabidopsis Regulator Plant Science Biology 01 natural sciences Hypocotyl 03 medical and health sciences chemistry.chemical_compound Cytochrome P-450 Enzyme System Plant Growth Regulators Gene Expression Regulation Plant Transcription (biology) ethylene 030304 developmental biology 0303 health sciences hyponasty Arabidopsis Proteins Ethylenes biology.organism_classification Cell biology Plant Leaves brassinosteroids ROTUNDIFOLIA3 Biochemistry chemistry Hyponastic response cell expansion Protein Binding Research Paper 010606 plant biology & botany |
| Zdroj: | Journal of Experimental Botany |
| ISSN: | 1460-2431 0022-0957 |
| DOI: | 10.1093/jxb/ers356 |
| Popis: | Upward leaf movement, called hyponastic growth, is employed by plants to cope with adverse environmental conditions. Ethylene is a key regulator of this process and, in Arabidopsis thaliana, hyponasty is induced by this phytohormone via promotion of epidermal cell expansion in a proximal zone of the abaxial side of the petiole. ROTUNDIFOLIA3/CYP90C1 encodes an enzyme which was shown to catalyse C-23 hydroxylation of several brassinosteroids (BRs) – phytohormones involved in, for example, organ growth, cell expansion, cell division, and responses to abiotic and biotic stresses. This study tested the interaction between ethylene and BRs in regulating hyponastic growth. A mutant isolated in a forward genetic screen, with reduced hyponastic response to ethylene treatment, was allelic to rot3. The cause of the reduced hyponastic growth in this mutant was examined by studying ethylene–BR interaction during local cell expansion, pharmacological inhibition of BR synthesis and ethylene effects on transcription of BR-related genes. This work demonstrates that rot3 mutants are impaired in local cell expansion driving hyponasty. Moreover, the inhibition of BR biosynthesis reduces ethylene-induced hyponastic growth and ethylene increases sensitivity to BR in promoting cell elongation in Arabidopsis hypocotyls. Together, the results show that ROT3 modulates ethylene-induced petiole movement and that this function is likely BR related. |
| Databáze: | OpenAIRE |
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