Real‐time polymerase chain reaction quantification of the salivary levels of cariogenic bacteria in patients with orthodontic fixed appliances
| Autor: | Maribasappa Karched, Eunice M. Pauline, Radhika G. Bhardwaj, Manal Abu Al-Melh |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Adult
DNA Bacterial Male medicine.medical_specialty Saliva Lactobacillus casei Adolescent Orthodontic Brackets Real-Time Polymerase Chain Reaction Gastroenterology Streptococcus salivarius Streptococcus sobrinus Bacterial Adhesion law.invention plaque Streptococcus mutans law Internal medicine Medicine Humans General Dentistry Polymerase chain reaction saliva biology business.industry Streptococcus gordonii Streptococcus Original Articles biology.organism_classification lcsh:RK1-715 Lactobacillus Real-time polymerase chain reaction lcsh:Dentistry Case-Control Studies Original Article Female real‐time PCR business |
| Zdroj: | Clinical and Experimental Dental Research Clinical and Experimental Dental Research, Vol 6, Iss 3, Pp 328-335 (2020) |
| ISSN: | 2057-4347 |
| Popis: | Aim The aim was to investigate the salivary detection frequencies and quantities of caries‐associated bacteria from patients with orthodontic brackets. Methods Patients wearing orthodontic brackets (n = 40, mean age = 26 years) and healthy controls without brackets (n = 40, mean age = 17 years) were enrolled in the study. Saliva samples from each patient was collected. After DNA purification, target species comprising streptococci and a Lactobacillus species were detected and quantified from the samples using polymerase chain reaction (PCR) and real‐time quantitative PCR. Results Detection frequencies did not differ between the orthodontic patients and the control subjects for any target species except for Streptococcus sobrinus, which showed significantly lower detection rates in orthodontic patients (p |
| Databáze: | OpenAIRE |
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