In vitro isolation and characterization of bovine Neospora caninum in Korea
| Autor: | Woo-Suk Hwang, Young-Hwa Jean, Hyun-Joo Sohn, Eui-Kyung Hwang, Jae-Hoon Kim, Dae Yong Kim, I Yamane |
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| Rok vydání: | 2000 |
| Předmět: |
Cattle Diseases
Polymerase Chain Reaction Neospora Antigen parasitic diseases medicine Animals Fluorescent Antibody Technique Indirect reproductive and urinary physiology Fetus Korea General Veterinary biology Coccidiosis fungi Aborted Fetus General Medicine medicine.disease biology.organism_classification Immunohistochemistry Virology Neospora caninum Blotting Southern Microscopy Electron embryonic structures Vero cell Cattle Parasitology Immunostaining |
| Zdroj: | Veterinary Parasitology. 90:147-154 |
| ISSN: | 0304-4017 |
| DOI: | 10.1016/s0304-4017(00)00224-7 |
| Popis: | The brains of nine aborted bovine fetuses and two newborn calves born from dams suspected to be infected with Neospora caninum were homogenized and inoculated into Vero cells. All fetuses and calves were from cows determined as seropositive to N. caninum by an IFA test. Sera and thoracic fluids of all fetuses and calves also revealed high antibody titer to N. caninum by IFAT ranging from 1:800 to 1:3200. N. caninum was isolated from the brains of one aborted fetus and one newborn calf when the brain homogenates were grown continuously in Vero cell culture. N. caninum tachyzoites, giemsa-positive, were first observed on Days 45 and 56 postinoculation in the newborn calf and the aborted fetus, respectively. The isolates (KBA-1 and KBA-2) were morphologically and ultrastructurally similar to previously published Neospora isolates. The isolated parasites were confirmed as N. caninum by means of the antigenic reactivities, immunostaining, PCR and southern blotting, and electron microscopy. |
| Databáze: | OpenAIRE |
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