Oxygen supplementation to limit hypoxia-induced muscle atrophy in C2C12 myotubes: comparison with amino acid supplement and electrical stimulation
| Autor: | Samir Bensaid, Claudine Fabre, Mehdi Pawlak-Chaouch, Caroline Cieniewski-Bernard |
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| Přispěvatelé: | Unité de Recherche Pluridisciplinaire Sport, Santé, Société (URePSSS) - ULR 7369 - ULR 4488 (URePSSS), Université d'Artois (UA)-Université du Littoral Côte d'Opale (ULCO)-Université de Lille, Université de Lille, Univ. Artois, Univ. Littoral Côte d’Opale, Unité de Recherche Pluridisciplinaire Sport, Santé, Société (URePSSS) - ULR 7369 - ULR 4488 [URePSSS], Unité de Recherche Pluridisciplinaire Sport, Santé, Société (URePSSS) - ULR 7369 |
| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: |
Histology
Myotube morphology [SDV]Life Sciences [q-bio] Muscle Fibers Skeletal Oxygen Inhalation Therapy Cell Biology Electrical stimulation Oxygenation treatment Protein homeostasis Skeletal muscle hypoxia Electric Stimulation Pathology and Forensic Medicine Oxygen Muscular Atrophy Phosphatidylinositol 3-Kinases Humans Amino Acids Hypoxia Muscle Skeletal |
| Zdroj: | Cell and Tissue Research Cell and Tissue Research, 2022, Cell and Tissue Research, ⟨10.1007/s00441-021-03492-x⟩ |
| DOI: | 10.1007/s00441-021-03492-x⟩ |
| Popis: | International audience; In skeletal muscle, chronic oxygen depletion induces a disturbance leading to muscle atrophy. Mechanical stress (physical exercise) and nutritional supplement therapy are commonly used against loss of muscle mass and undernutrition in hypoxia, while oxygenation therapy is preferentially used to counteract muscle fatigue and exercise intolerance. However, the impact of oxygenation on skeletal muscle cells remains poorly understood, in particular on signalling pathways regulating protein balance. Thus, we investigated the effects of each separated treatment (mechanical stress, nutritional supplementation and oxygenation therapy) on intracellular pathways involved in protein synthesis and degradation that are imbalanced in skeletal muscle cells atrophy resulting from hypoxia. Myotubes under hypoxia were treated by electrical stimulation, amino acids supplement or oxygenation period. Signalling pathways involved in protein synthesis (PI3K-Akt-mTOR) and degradation (FoxO1 and FoxO3a) were investigated, so as autophagy, ubiquitin-proteasome system and myotube morphology. Electrical stimulation and oxygenation treatment resulted in higher myotube diameter, myogenic fusion index and myotubes density until 48 h post-treatment compared to untreated hypoxic myotubes. Both treatments also induced inhibition of FoxO3a and decreased activity of ubiquitin-proteasome system; however, their impact on protein synthesis pathway was specific for each one. Indeed, electrical stimulation impacted upstream proteins to mTOR (i.e., Akt) while oxygenation treatment activated downstream targets of mTOR (i.e., 4E-BP1 and P70S6K). In contrast, amino acid supplementation had very few effects on myotube morphology nor on protein homeostasis. This study demonstrated that electrical stimulation or oxygenation period are two effective treatments to fight against hypoxia-induced muscle atrophy, acting through different molecular adaptations. |
| Databáze: | OpenAIRE |
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