The nuclear receptor liver receptor homolog-1 is an estrogen receptor target gene
| Autor: | Nadège Servant, Vincent Cavaillès, Anne Licznar, Jean-Sébastien Annicotte, Gwendal Lazennec, Pascal Pujol, Françoise Vignon, Aurélie Bardin, Thierry Maudelonde, Carine Chavey, Jacques Teyssier, Lluis Fajas, Eric Badia |
|---|---|
| Přispěvatelé: | Le Ster, Yves, Endocrinologie moléculaire et cellulaire des cancers, Université Montpellier 1 (UM1)-Institut National de la Santé et de la Recherche Médicale (INSERM), Muscle et pathologies, Université Montpellier 1 (UM1)-IFR3, Université Montpellier 1 (UM1)-Université Montpellier 1 (UM1)-Institut National de la Santé et de la Recherche Médicale (INSERM), Laboratoire de Biologie Cellulaire et Hormonale, Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier)-Hôpital Arnaud de Villeneuve |
| Jazyk: | angličtina |
| Rok vydání: | 2005 |
| Předmět: |
Cancer Research
Transcription Genetic Estrogen receptor Receptors Cytoplasmic and Nuclear Estrogen-related receptor alpha 0302 clinical medicine E2 Nuclear receptors MESH: RNA Small Interfering RNA Small Interfering Promoter Regions Genetic MESH: Estrogen Receptor alpha Nuclear receptor co-repressor 1 0303 health sciences MESH: Gene Expression Regulation Neoplastic Cell biology DNA-Binding Proteins Gene Expression Regulation Neoplastic MESH: Promoter Regions (Genetics) 030220 oncology & carcinogenesis Interleukin-21 receptor Disease Progression Female MESH: Disease Progression Transcription medicine.medical_specialty MESH: Cell Line Tumor MESH: Receptors Cytoplasmic and Breast Neoplasms [SDV.CAN]Life Sciences [q-bio]/Cancer Biology Article 03 medical and health sciences [SDV.CAN] Life Sciences [q-bio]/Cancer Internal medicine Cell Line Tumor Breast Cancer Genetics medicine [SDV.BBM] Life Sciences [q-bio]/Biochemistry Molecular Biology Humans [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology Molecular Biology Estrogen receptor beta 030304 developmental biology Binding Sites MESH: Humans Liver receptor homolog-1 Estrogen Receptor alpha Ftz-F1 Endocrinology MESH: Binding Sites Estrogen-related receptor gamma Gene expression Estrogen receptor alpha MESH: Female LRH-1 MESH: Breast Neoplasms MESH: DNA-Binding Proteins Transcription Factors |
| Zdroj: | Oncogene Oncogene, Nature Publishing Group, 2005, 24 (55), pp.8167-75. ⟨10.1038/sj.onc.1208950⟩ |
| ISSN: | 0950-9232 1476-5594 |
| DOI: | 10.1038/sj.onc.1208950⟩ |
| Popis: | International audience; Liver receptor homolog-1 (LRH-1) is a nuclear receptor previously known to have distinct functions during mouse development and essential roles in cholesterol homeostasis. Recently, a new role for LRH-1 has been discovered in tumor progression, giving LRH-1 potential transforming functions. In order to identify critical factors stimulating LRH-1 expression leading to deregulated cellular proliferation, we studied its expression and its regulation in several breast cancer cell lines. We observed that LRH-1 expression was increased in estrogen receptor (ER) alpha expressing cell lines, whereas weak-to-no expression was found in nonexpressing ERalpha cell lines. In MCF7, LRH-1 expression was highly induced after treatment with 17beta-estradiol (E2). This transcriptional regulation was the result of a direct binding of the ER to the LRH-1 promoter, as demonstrated by gelshift and chromatin immunoprecipitation assays. Interestingly, siRNA-mediated inactivation of LRH-1 decreased the E2-dependent proliferation of MCF7 cells. Finally, LRH-1 protein expression was detected by immunohistochemistry in tumor cells of human mammary ductal carcinomas. Altogether, these data demonstrate that LRH-1 is transcriptionally regulated by the ER alpha and reinforce the hypothesis that LRH-1 could exert potential oncogenic effects during breast cancer formation. |
| Databáze: | OpenAIRE |
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