Specific Versus Non‐specific Response in Exponential Molecular Amplification from Cross‐Catalysis: Modeling the Influence of Background Amplifications on the Analytical Performances
| Autor: | Corentin Calvet, François Mavré, Mathieu Branca, Benoît Limoges |
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| Přispěvatelé: | Laboratoire d'Electrochimie Moléculaire (LEM (UMR_7591)), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université de Paris (UP) |
| Rok vydání: | 2021 |
| Předmět: |
Basis (linear algebra)
Kinetic model Computer science [CHIM.CATA]Chemical Sciences/Catalysis 02 engineering and technology 010402 general chemistry 021001 nanoscience & nanotechnology 01 natural sciences Signal Stability (probability) Instability Atomic and Molecular Physics and Optics 0104 chemical sciences Exponential function Non specific [CHIM.ANAL]Chemical Sciences/Analytical chemistry [CHIM]Chemical Sciences Intrinsic instability Physical and Theoretical Chemistry 0210 nano-technology Biological system |
| Zdroj: | ChemPhysChem ChemPhysChem, Wiley-VCH Verlag, 2021, ⟨10.1002/cphc.202100342⟩ |
| ISSN: | 1439-7641 1439-4235 |
| Popis: | International audience; Molecular based signal amplifications relying on an autocatalytic process may represent an ideal strategy for the development of ultrasensitive analytical or bioanalytical assays, the main reason being the exponential nature of the amplification. However, to take fully advantage of such amplification rates, high stability of the starting co-reactants is required in order to avoid any undesirable background amplification. Here, on the basis of a simple kinetic model of cross-catalysis including a certain degree of intrinsic instability of co-reactants, we highlight the key parameters governing the analytical response of the system and discuss the analytical performances that are expected from a given kinetic set. In particular, we show how the detection limit is directly related to the relative instability of reactants within each catalytic loop. The model is validated with an experimental dataset and is intended to serve as a guide in the design and optimization of autocatalytic molecularbased amplification systems with improved analytical performances. |
| Databáze: | OpenAIRE |
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