Development and validation of a UPLC-ESI-MS method for quantitation of the anti-Alzheimer drug galantamine and other Amaryllidaceae alkaloids in plants

Autor: Ana Sheila de Queiroz Souza, José Régis de Paiva, Kirley Marques Canuto, Guilherme Julião Zocolo, Otília Deusdênia L. Pessoa, Rita de Cássia Alves Pereira, Paulo Riceli Vasconcelos Ribeiro, Edy Sousa de Brito
Přispěvatelé: JOSÉ RÉGIS DE PAIVA, Departamento de Química, Universidade Federal do Ceará, Campus do Pici, ANA SHEILA DE QUEIROZ SOUZA, Departamento de Química, Universidade Federal do Ceará, Campus do Pici, RITA DE CASSIA ALVES PEREIRA, CNPAT, PAULO RICELI VASCONCELOS RIBEIRO, CNPAT, GUILHERME JULIAO ZOCOLO, CNPAT, EDY SOUSA DE BRITO, CNPAT, OTÍLIA DEUSDÊNIA LOIOLA PESSOA, Departamento de Química, Universidade Federal do Ceará, Campus do Pici, KIRLEY MARQUES CANUTO, CNPAT.
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Zdroj: Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA-Alice)
Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
Popis: Our study aimed at the development of a method based on ultra-performance liquid chromatography coupled to mass spectrometry (UPLC-ESI-MS) using selected ion monitoring for the simultaneous quantitation of the alkaloids galantamine, pseudolycorine, sanguinine and narciclasine in Amaryllidaceae species (Hippeastrum elegans, Habranthus cf. irwinianus, Hymenocallis littoralis and Griffinia nocturna). The alkaloids were extracted from dried and ground bulbs (100 mg) using liquid-liquid microextraction followed by solid phase extraction in cation exchange cartridges. The quantification method showed good linearity (correlation coefficient, R ≥ 0.9968) and selectivity for a run time of 10 min. All values were within the acceptable limits for recovery (87.5-96.2%), interday (coefficient of variation, CV%, 1.3-8.4%) and intraday precision (CV% 5.7-8.1%), except narciclasine (70%). The limits of detection and quantification were 5-100 and 20-350 ng mL-1, respectively. Our method demonstrated to be rapid, sensitive and reliable, therefore appropriate for being employed in the prospection of new source of galantamine and other alkaloids.
Databáze: OpenAIRE
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