ATP driven clathrin dependent entry of carbon nanospheres prefer cells with glucose receptors
| Autor: | Tapas K. Kundu, Dinesh Jagadeesan, Piyush Chaturbedy, Muthusamy Eswaramoorthy, B. S. Suma, Ruthrotha B. Selvi, Snehajyoti Chatterjee |
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| Jazyk: | angličtina |
| Předmět: |
Sucrose
lcsh:Medical technology lcsh:Biotechnology Biomedical Engineering Medicine (miscellaneous) Pharmaceutical Science Receptors Cell Surface Bioengineering Deoxyglucose Endocytosis Blood–brain barrier Clathrin Applied Microbiology and Biotechnology chemistry.chemical_compound Mice Adenosine Triphosphate lcsh:TP248.13-248.65 Cell Line Tumor parasitic diseases medicine Animals Humans Brain Chemistry Mice Inbred BALB C Microscopy Confocal biology Research Glucose transporter Temperature Brain Receptor-mediated endocytosis Carbon Cell biology medicine.anatomical_structure chemistry lcsh:R855-855.5 Cell culture Cancer cell biology.protein NIH 3T3 Cells Molecular Medicine Adenosine triphosphate Nanospheres HeLa Cells |
| Zdroj: | Journal of Nanobiotechnology Journal of Nanobiotechnology, Vol 10, Iss 1, p 35 (2012) |
| ISSN: | 1477-3155 |
| DOI: | 10.1186/1477-3155-10-35 |
| Popis: | Background Intrinsically fluorescent glucose derived carbon nanospheres (CSP) efficiently enter mammalian cells and also cross the blood brain barrier (BBB). However, the mechanistic details of CSP entry inside mammalian cells and its specificity are not known. Results In this report, the biochemical and cellular mechanism of CSP entry into the living cell have been investigated. By employing confocal imaging we show that CSP entry into the mammalian cells is an ATP-dependent clathrin mediated endocytosis process. Zeta potential studies suggest that it has a strong preference for cells which possess high levels of glucose transporters such as the glial cells, thereby enabling it to target individual organs/tissues such as the brain with increased specificity. Conclusion The endocytosis of Glucose derived CSP into mammalian cells is an ATP dependent process mediated by clathrin coated pits. CSPs utilize the surface functional groups to target cells containing glucose transporters on its membrane thereby implicating a potential application for specific targeting of the brain or cancer cells. |
| Databáze: | OpenAIRE |
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