Ionotropic glutamatergic neurotransmission in the ventral tegmental area modulates ΔFosB expression in the nucleus accumbens and abstinence syndrome in morphine withdrawal rats
| Autor: | Dong-Yuan Cao, Hui-Sheng Wang, Xiao-Hui Xiang, Yuan Guo, Wei-Ran Wu, Hui-Ling Wang, Yan Zhao |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Male
medicine.medical_specialty Microinjections Physical dependence (+)-Naloxone Nucleus accumbens Receptors N-Methyl-D-Aspartate Synaptic Transmission Nucleus Accumbens Rats Sprague-Dawley chemistry.chemical_compound Quinoxalines Internal medicine Basal ganglia medicine DNQX Animals Protein Isoforms Pharmacology Morphine Naloxone Kindling Chemistry Ventral Tegmental Area Immunohistochemistry Rats Substance Withdrawal Syndrome Ventral tegmental area Endocrinology medicine.anatomical_structure Dizocilpine Maleate medicine.symptom Excitatory Amino Acid Antagonists Morphine Dependence Proto-Oncogene Proteins c-fos Ionotropic effect |
| Zdroj: | European Journal of Pharmacology. 527:94-104 |
| ISSN: | 0014-2999 |
| DOI: | 10.1016/j.ejphar.2005.10.017 |
| Popis: | The present study sought to assess whether the blockade of ionotropic glutamate receptors in the ventral tegmental area could modulate morphine withdrawal in morphine-dependent rats and the expression of stable DeltaFosB isoforms in the nucleus accumbens during morphine withdrawal. Rats were injected (i.p.) with increasing doses of morphine for 1 week to develop physical dependence, and withdrawal was then precipitated by one injection of naloxone (2 mg/kg, i.p.). Abstinence signs such as jumping, wet-dog shake, writhing posture, weight loss, and Gellert-Holtzman scale score were recorded to evaluate naloxone-induced morphine withdrawal. Two ionotropic glutamate receptor antagonists, dizocilpine (MK-801) and 6, 7-dinitroquinnoxaline-2, 3-dione (DNQX), were microinjected unilaterally into the ventral tegmental area 30 min before naloxone precipitation. A second injection of naloxone (2 mg/kg i.p.) was given 1 h after the first naloxone injection to sustain a maximal level of withdrawal so that the expression of stable DeltaFosB isoforms in the nucleus accumbens could be measured. This would enable determination of the correlation between the MK-801 or DNQX-induced decrease in somatic withdrawal signs and the change in neuronal activity in the nucleus accumbens. The results showed that both MK-801 and DNQX significantly alleviated all symptoms of morphine withdrawal except for weight loss and reduced the expression of stable DeltaFosB isoforms within the nucleus accumbens. These data suggest that ionotropic glutamatergic neurotransmission in the ventral tegmental area regulates the levels of stable DeltaFosB isoforms in the nucleus accumbens, which play a very important role in modulating opiate withdrawal. |
| Databáze: | OpenAIRE |
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