The peptidyl-puromycin reaction with Neurospora crassa ribosomes
| Autor: | F. A. M. Alberghina, E. Sturani, L. Schiaffonati |
|---|---|
| Rok vydání: | 1971 |
| Předmět: |
Sucrose
Time Factors Phenylalanine cytology/growth /&/ development Neurospora cytology/growth /&/ development Phenylalanine Protein Biosynthesis Puromycin RNA Ribosomal Ribosomes Sucrose Time Factors Tritium Tritium Biochemistry Microbiology Ribosome Neurospora crassa chemistry.chemical_compound Genetics Protein biosynthesis Molecular Biology Ribosomal biology Chemistry fungi General Medicine Ribosomal RNA biology.organism_classification In vitro RNA Ribosomal Puromycin Protein Biosynthesis RNA Ribosomes |
| Zdroj: | Archiv f�r Mikrobiologie. 80:166-175 |
| ISSN: | 1432-072X 0302-8933 |
| DOI: | 10.1007/bf00411881 |
| Popis: | The reaction of the peptidyl-tRNA in an “in vitro” system from Neurospora crassa with puromycin has been studied by different experimental approaches. Ribosomes precharged with labelled polyphenylalanine have been prepared and the release of radioactivity, occurring after the reaction with puromycin, has been followed on sucrose density gradients. Furthermore the reaction of endogenous peptidyl-tRNA carried by ribosomes isolated from actively growing mycelia with 3H-puromycin has been characterized. By this latter technique it has been possible to evaluate the percentage of ribosomes engaged in protein synthesis in ribosomal populations isolated from mycelia in different stages of growth. |
| Databáze: | OpenAIRE |
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