Tyrosinase as a dual reporter gene for both photoacoustic and magnetic resonance imaging
| Autor: | Mary M. Hitt, Robert J. Paproski, Roger J. Zemp, Keith Wachowicz, Alexander Forbrich |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
Pathology
medicine.medical_specialty Tyrosinase ocis:(000.1430) Biology and medicine 01 natural sciences 010309 optics Melanin 03 medical and health sciences Functional Imaging In vivo 0103 physical sciences medicine 030304 developmental biology 0303 health sciences Reporter gene medicine.diagnostic_test Chemistry ocis:(170.2655) Functional monitoring and imaging Cell migration Magnetic resonance imaging Transfection Atomic and Molecular Physics and Optics ocis:(170.5120) Photoacoustic imaging Biophysics Molecular imaging ocis:(170.3880) Medical and biological imaging Biotechnology |
| Zdroj: | Biomedical Optics Express |
| ISSN: | 2156-7085 |
| Popis: | Reporter genes are useful scientific tools for analyzing promoter activity, transfection efficiency, and cell migration. The current study has validated the use of tyrosinase (involved in melanin production) as a dual reporter gene for magnetic resonance and photoacoustic imaging. MCF-7 cells expressing tyrosinase appear brown due to melanin. Magnetic resonance imaging of tyrosinase-expressing MCF-7 cells in 300 μL plastic tubes displayed a 34 to 40% reduction in T1 compared to normal MCF-7 cells when cells were incubated with 250 μM ferric citrate. Photoacoustic imaging of tyrosinase-expressing MCF-7 cells in 700 μm plastic tubes displayed a 20 to 57-fold increase in photoacoustic signal compared to normal MCF-7 cells. The photoacoustic signal from tyrosinase-expressing MCF-7 cells was significantly greater than blood at 650 nm, suggesting that tyrosinase-expressing cells can be differentiated from the vasculature with in vivo photoacoustic imaging. The imaging results suggest that tyrosinase is a useful reporter gene for both magnetic resonance and photoacoustic imaging. |
| Databáze: | OpenAIRE |
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